BIOD 17lab 9 exam.docx

Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. Given Culture A and Culture B were accidentally mixed, what observations were made from the Gram stain (s ...

Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. Given Culture A and Culture B were accidentally mixed, what observations were made from the Gram stain (shown below)? Your Answer: Gram-positive cocci (spherical) clusters and Gram-negative bacillus (rods) were observed. Gram-positive cocci (spherical) clusters and Gram-negative bacillus (rods) were observed. Question 2 Not yet graded / 6 pts Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. Given the results from a MacConkey agar plate shown below, what is the shape of Culture A? Culture B? This study source was downloaded by 100000855457697 from CourseHero.com on 11-01-2022 02:58:06 GMT -05:00 https://www.coursehero.com/file/94661412/BIOD-171-lab-9-examdocx/ Your Answer: MacConkey agar is selective for Gram-negative bacteria. Thus, Culture A must be Gram positive as no growth was observed. Coupled to the results of the gram stain, The Culture A (Gram-positive) must be the cocci. Culture B must be Gram-negative (growth observed) and this correlates to the bacillus or rod-shaped bacteria. MacConkey agar is selective for Gram-negative bacteria. Thus, Culture A must be Gram-positive as no growth was observed. Coupled to the results of the gram stain, Culture A (Gram-positive) must be the cocci. Culture B must be Gram-negative (growth observed) and this correlates to the bacillus or rod-shaped bacteria. Question 3 Not yet graded / 6 pts Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. The hemolytic properties of both cultures were next examined by streaking each culture on a blood agar plate (shown below). What is the hemolytic status of Culture A? Culture B? This study source was downloaded by 100000855457697 from CourseHero.com on 11-01-2022 02:58:06 GMT -05:00 https://www.coursehero.com/file/94661412/BIOD-171-lab-9-examdocx/ Your Answer: Culture A exhibits beta hemolysis as defined by the region of clearing around the growing bacterial streak. Culture B exhibits gamma-hemolysis (non-hemolytic) as indicated by the white colonies growing on the still red agar. Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. Culture A exhibits beta hemolysis as defined by the region of clearing around the growing bacterial streak. Culture B exhibits gamma-hemolysis (non-hemolytic) as indicated by the white colonies growing on the still red agar. Question 4 Not yet graded / 6 pts Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. Culture A and Culture B were assessed via the catalase test as shown below. State your observations for each culture. This study source was downloaded by 100000855457697 from CourseHero.com on 11-01-2022 02:58:06 GMT -05:00 https://www.coursehero.com/file/94661412/BIOD-171-lab-9-examdocx/ Your Answer: Culture A and B were both catalase positive meaning both are able to breakdown hydrogen peroxide (damages cellular integrity) into harmless hydrogen and water molecules. Culture A and B were both catalase positive meaning both are able to breakdown hydrogen peroxide (damages cellular integrity) into harmless hydrogen and water molecules. Question 5 Not yet graded / 6 pts Using the observations made and recorded from PL09 Unknown Pathogen, complete the results sections accordingly. Culture A and Culture B were assessed via the oxidase test as shown below. State y