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WGU 785 Final Exam

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WGU 785 Final Exam Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome for their kids? ANS His daughters would be carriers. This is x-link recessive. Autosomal: Domi ... nant: ANS Autosomal: males and females equally affected. Dominant: non-carrier parents polymerase chain reaction (PCR) ANS The process of copying DNA in the lab. Uses Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA primers. 3 Steps of PCR ANS 1. Denaturation: DNA is heated to 95C to separate it. 2. Annealing: reaction is cooled to 50C; primers stick to the DNA you want to copy and add DNA polymerase. 3. Elongation: reaction heated to 70C and DNA polymerase, adding nucleotides building a new DNA strand. Base Excision Repair (BER) ANS How you repair a mutation. BER is used to repair damage to a base caused by harmful molecules. You remove the base that is damaged and replace it. *BER removes a single nucleotide* DNA glycolsylase - sees damaged DNA and removes it. DNA polymerase-puts the right one back in while DNA ligase seals it. Mismatch repair (MMR) occurs during: ANS replication. DNA polymerase proofreads but sometimes a mismatch pair gets through. MMR removes a large section of the nucleotides from the new DNA and DNA polymerase tries again. (Ex: C-T instead of C-A) Mismatch Repair corrects what kind of DNA damage? ANS When a base is mismatched due to errors in replication. Such as G-T instead of G-C. DNA polymerase comes by and fixes it. What happens when DNA polymerase binds to DNA to make RNA? ANS TRANSCRIPTION! DNA polymerase takes the individual nucleotides and matches them to the parental sequences to ensure a correct pair. It must bind with RNA primer to work. What is needed for DNA replication? ANS DNA polymerase [Show More]

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