Biology > QUESTIONS & ANSWERS > Westfield State University BIOL 0300Module_2_Transcription_Part_I_Answer_Sheet. (All)
MODULE 2: TRANSCRIPTION PART I ANSWER SHEET Q1. What is the span — the start and end base positions — the tra-RA transcription unit? The Span of the tra-RA transcription unit is 9,850-10,850 ... Q2. How do the peaks in the RNA-Seq Read Coverage track relate to mRNA abundance? The peaks in the RNA seq read coverage tracks related to the exons that are present , which means that parts are transcribes into mRNA. Summary Question 1. Most of the RNA-Seq reads come from mature (processed) RNA. Can you use this data to suggest where introns are located? Are there any regions that seem ambiguous? Introns are noncoding sections of a RNA transcript, that are spliced out before the RNA molecule is translated into a protein. Introns are also referred to as intervening sequences. Q3. Examine the "RNA-Seq Coverage" and the "FlyBase Genes" tracks in the Genome Browser from left to right. At approximately which coordinate (base position) does the RNA-Seq data start? Remember that you can use the navigation controls at the top of the page to zoom in to the region of interest. The RNA-Seq data start at 9900. Q4. How many TSS sites were identified using this technique? There were total two TSS sites were identified using this technique [Show More]
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