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BioChem PA
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PCR o Polymerase Chain Reaction o What do we need for PCR Target DNA dNTPs = deoxnucleotides DNA Primers TAQ Polymerase = Enzyme “Ase” Stable at high temperatures o... Steps for PCR 1. Denaturation Sample is heated to 95 degrees C to separate the 2 DNA strands 2. Annealing = “Sticks” Next, cooled to 50 degrees C. Primers bind (base pair) to the template DNA 3. Elongation DNA Polymerase works well at 70 degrees C- DNA Polymerase synthesizes new strands of DNA starting at the primers Typical there will be about 30 rounds of PCR 2n = # DNA molecules produces o 2-1 = 2 o 2-2 =4 [Show More]
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