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Summary Microbiology task 1.docx.docx

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Introduction: Gram-positive and Gram-negative bacteria have a big difference in cell wall structure that is examined during the gram staining lab. Gram-positive bacteria have thick layers of peptido ... glycan. When these bacteria are stained, using a crystal violet dye, they will remain purple. Gram-negative bacteria have thin peptidoglycan walls, which do not retain the purple dye. It must be counterstained with safranin turning them pink. Materials and Methods: There are four basic steps for gram staining. The first step is to flood the cells with crystal violet dye for 1 minute and then rinse. Step 2 is to put iodine on the cells so that the violet dye sticks more. [Show More]

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