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St. Clair College BIO 206OSX_Microbiology_TestBank_Ch12.

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OpenStax Microbiology Test Bank Chapter 12: Modern Applications of Microbial Genetics Chapter 12: Modern Applications of Microbial Genetics * = Correct answer Multiple Choice 1. Which of the following is the definition of biotechnology? A. The alteration of DNA in a cell B. The use of living systems to benefit humankind* C. Using computers to synthesize biomolecules D. Using DNA for solving mathematical problems Difficulty: Easy ASM Standard: 26 2. Which of the following is the definition of genetic engineering? A. The alteration of DNA in a cell* B. The use of living systems to benefit humankind C. Using computers to synthesize biomolecules D. Using DNA for solving mathematical problems Difficulty: Easy ASM Standard: 15, 19, 26, 31 3. The first set of genes to be introduced into E. coli came from which of the following? A. Arabidopsis B. Haemophilus C. Mycobacterium D. Xenopus* Difficulty: Easy ASM Standard: 15, 19 4. Which of the following is a palindrome sequence that could be recognized by a restriction enzyme? A. 5′ – GAAAAG – 3′ 3′ – CTTTTC – 5′ B. 5′ – GAATTC – 3′ * 3′ – CTTAAG – 5′ C. 5′ – GACT – 3′ 3′ – CTGA – 5′ D. 5′ – GCCCC – 3′ 3′ – CGGGG – 5′ Difficulty: Moderate ASM Standard: 26, 36 Page 1 of 17 OpenStax Microbiology Test Bank Chapter 12: Modern Applications of Microbial Genetics 5. Restriction enzymes (endonucleases) are produced by bacteria as a means of protection against which infectious agent? A. antibiotics B. antibodies C. bacteriophages* D. prions Difficulty: Easy ASM Standard: 18, 26 6. Which of the following enzymes rejoins two sugar-phosphate backbones of DNA? A. helicase B. ligase* C. restriction enzyme D. reverse transcriptase Difficulty: Easy ASM Standard: 26, 36 7. Genes to be cloned into a plasmid are typically inserted in which location? A. in a location disrupting the antibiotic selective marker B. oriC (origin of replication) C. polylinker site* D. randomly Difficulty: Easy ASM Standard: 19, 26, 36

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