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CCBC Microbiology 230-Summer 2022- Quiz #2

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CCBC Microbiology 230-Summer 2022- Quiz #2 1. Why do we heat fix the bacteria to the slide before staining? - ✔✔So the bacteria will hold the stain better. 2. A basic dye has the color in the ... _____________ ion - ✔✔Positive 3. All bacteria have a slight negative charge; a basic dye has the color in the positive ion. Opposite charges attract. This is the principle behind: - ✔✔direct staining 4. Why do gram positive bacteria appear purple after staining? - ✔✔The alcohol and acetone dehydrates the many layers of peptidoglycan 5. A bacterial that has one flagella is called what? - ✔✔Monotrichis. 6. A bacteria that has flagella all around its outside coat (surrounded by flagella) is called what? - ✔✔Peritrichas. 7. A bacteria that has a flagella that is coming out both ends is called what? - ✔✔Lophotricus. 8. A bacteria that has a single flagella at both poles is called what? - ✔✔Amphitrichous. 9. What are the steps for staining a capsul? - ✔✔1) put five loops of bacteria on a slide from a broth mixture 2) Let mixture dry on slide. 3) add two drops of crystal violet, spread on slide. 4) let slide dry for 1-2 min. 5) flood slide with copper sulfate let sit for 20 seconds. 6) Shake off excess copper sulfate.7) Blot slide dry. [Show More]

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