BIOS 390 ERIC_KYLE_Week_5_Exam_Study_Guide with complete questions and answers

BIOS 390 ERIC_KYLE_Week_5_Exam_Study_Guide with complete questions and answers 2020 BIOS390 Week 5 Exam Study Guide Question Set 1 (TCO 6) A major difference between transcription in bacteria and eukaryotes is (T ...

BIOS 390 ERIC_KYLE_Week_5_Exam_Study_Guide with complete questions and answers 2020 BIOS390 Week 5 Exam Study Guide Question Set 1 (TCO 6) A major difference between transcription in bacteria and eukaryotes is (TCO 6) Transcription is the process (TCO 6) Transcription proceeds in the (what direction?): Question Set 2 (TCO 6) Define promoter: (TCO 6) Define promoter strength: (TCO 6) Promoter is located (Chapter 10, page 264 Question Set 3 (TCO 6) Which of the following statements is correct? A. DNA polymerase requires a primer to get started. B. RNA polymerase requires a primer to get started. C. Both DNA and RNA polymerase require primers to get started. D. Both DNA and RNA polymerase can start synthesis de novo. (TCO 6) Which statement is not true about the bacterial sigma factor? A. Sigma is a regulatory protein, which is required for the initiation of transcription. B. Sigma has the ability to join ribonucleotides (NTPs) by phosphodiester bonds using DNA as a template. C. Sigma properly orients the RNA polymerase complex for transcription at the gene start site. D. After initiation is complete, sigma does not dissociate completely; some domains are displaced and mRNA synthesis continues. (TCO 6) RNA polymerase forms a phosphodiester bond between. Question Set 4 (TCO 6) Promoter clearance refers to a step of transcription initiation in which (TCO 6) Which statement regarding bacterial Rho is not true? A. Rho is a helicase. B. Rho binds a specific C-rich sequence called a rut site. C. Rho-dependent transcriptional termination depends on the ability of Rho to access the mRNA. D. Rho rides along with RNA polymerase until a termination sequence is encountered. (TCO 6) Rho-independent terminators have all of the following characteristics except: Question Set 5 (TCO 6) Full induction of the lac operon occurs when (TCO 6) The real inducer of the lac operon (TCO 6) CAP is said to be responsible for positive regulation of the lac operon because: Question Set 6 (TCO 6) Transcription of protein-coding genes in eukaryotes is carried out (TCO 6) Which of the following is involved in transcription in prokaryotes, but not in eukaryotic transcription? (TCO 6) Gene regulatory elements are A. trans-acting transcription factors. B. cis-acting transcription factors. C. trans-acting DNA sequences D. cis-acting DNA sequences Question Set 7 (TCO 6) The unwinding of DNA during the initiation of transcription is mediated by the helicase activity of (TCO 6) Reinitiation of transcription requires (TCO 6) Elongation of transcription requires Question Set 8 (TCO 7) The ovalbumin gene is much longer than the mRNA produced by this gene. What causes this discrepancy? (TCO 7) RNA splicing is the process by which introns are removed from a primary RNA transcript at precisely defined splice points. Which statement is not true about introns? A. Introns are joined together after splicing. B. Introns are usually nonfunctional and are degraded, but some introns encode functional RNA products C. Introns are transcribed along with the exons. D. Introns are larger on average than exons. (TCO 7) Some introns contain termination codons, yet they do not interrupt the coding of a particular protein. Why? Question Set 9 (TCO 7) Select three posttranscriptional modifications often seen in the maturation of nuclear pre-mRNA in eukaryotes. (TCO 7) During maturation of nuclear pre-mRNA in eukaryotes, capping takes place at (TCO 7) Which is not a function of the 7-methylguanosine cap? A. Protection from exonucleases B. Promoting mRNA nuclear export C. Stimulation of RNA polymerase elongation D. Stimulation of translation Question Set 10 (TCO 7) The process of polyadenylation is accomplished by (TCO 7) RNA editing is a widespread mechanism for post-transcriptional modification of the base sequence of mRNa. In humans, (TCO 7) MicroRNAs are processed by the enzymes _____ and _____. One strand of the microRNA is loaded into a silencing complex named _____. The microRNAs target mRNA for _____ or _____. Question Set 11 (TCO 7) When mRNAs lacking a poly(A) tail compete with polyadenylated mRNAs for limiting translational machinery, (TCO 7) The nuclear exosome (TCO 7) RNA interference is thought to have evolved to Question Set 12 (TCO 6) “When glucose is abundant, bacteria use it exclusively as their food source, even when other sugars are present.” Discuss this statement in the context of how the presence of glucose effects induction of the lac operon. (TCO 6) For bacterial operon induction, describe a specific example of the following: “The repressor and activators are DNA binding proteins that undergo allosteric modification.” (TCO 6) You include an inhibitor of the protein kinase activity of TFIIH in an in vitro transcription assay. What step in transcription would you expect to see blocked?