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ASCP- Clinical Chemistry| 126 question| with complete solutions

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Diurnal variation Correct Answer: Changes in concentration of an analyte based on the time of the day (measured at day vs. night time) What is increased in the AM? (b/c of diurnal variation) Corre ... ct Answer: ACTH, Cortisol, Iron ACTH role Correct Answer: adrenocorticotropic hormone- stimulates adrenal cortex to secrete CORTISOL How does stress affect chemistry values? Correct Answer: Increase in ACTH, cortisol, and catecholamines Catecholamines Correct Answer: Hormones made by adrenal glands during stress. Ex. dopamine, epinephrine, norepinephrine Which analyte values are affected if delay in processing b/c serum/plasma is not separated in time? Correct Answer: Ammonia, lactic acid, K, Mg, LD, glucose Components of Spectrophotometry (in order) Correct Answer: Light source- monochromator- cuvette- photodetector- readout Fluorometry components (in order) Correct Answer: Light source- primary monochromator- cuvette- secondary monochromator- detector- readout When is thin-layer chromatography primarily used? Correct Answer: Screening test for drugs of abuse in urine When is high-performance liquid chromatography (HPLC) used? Correct Answer: Separation of thermolabile compounds When is gas chromatography used? Correct Answer: Separation of volatile compounds (therapeutic & toxic drugs) what is Glucose and when does it decrease Correct Answer: major source of cellular energy. Levels decrease if sample sits at RT. What are the common methods to measure glucose? Correct Answer: Glucose oxidase & hexokinase= most common (hexokinase is more accurate) Is cholesterol a risk factor for coronary artery disease (CAD)? Correct Answer: not by itself (must be analyzed with HDL & LDL) HDL Correct Answer: "good fat"- levels should b [Show More]

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