Health Care > EXAM > SCNM Q3 Microbiology Lab – Final| QUESTIONS WITH COMPLETE SOLUTIONS (All)

SCNM Q3 Microbiology Lab – Final| QUESTIONS WITH COMPLETE SOLUTIONS

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Gel Electrophoresis is a technique used to separate Correct Answer: DNA fragments and other macromolecules by size and charge DNA samples are loaded into wells/indentations at one end of a gel and... an electric current is applied to pull them through the gel DNA fragments are negatively charged, so they move towards the positive electrode Because all DNA fragments have the same amount of charge per mass, small fragments move through th egel faster than large ones In Gel Electrophoresis, when a gel is stained with a DNA-binding dye, the DNA fragments can be seen as Correct Answer: Bands --> each representing a group of same-sized DNA fragments In Gel Electrophoresis, a sample that matches up with that specific band is a (positive/negative) for that result Correct Answer: POSITIVE What are the differences between a Gram (+) and Gram (-) stain? (how do they appear on a slide?) Correct Answer: They are differentiated by: Size, Cell Wall, and Shape (rods or cocci) Gram (+) = Violet/Blue +Cocci = purple- grape-like clusters +S. Aureus +(Peptidoglycans linked together) +THICK cell walls = absorb stain = VIOLET Gram (-) = RED -Rod -E. Coli -(Outermembrane - lipoproteins - peptidoglycan - space - cytoplasmic membrane) -THIN cell walls = don't absorb much stain, but WILL absorb the LAST stain = (Ex. RED SAFRANIN) Which organisms are Gram (+)? Correct Answer: +Streptococcus Pneumoniae +Staphylococcus Aureus +Enterococcus + + + Which organisms are Gram (-)? Correct Answer: -Enterobacteriacea (E. coli, Klebsiella spp, enterobacter) [Show More]

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