ASCP MB Questions and Answers (Complete Solution) Graded A+

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ASCP MB Questions and Answers (Complete Solution) Graded A+ 1 / 31 1. What is biotin and digoxigenin most commonly used for in the lab? Why are they safer?: Probe labeling (aka tagging nucleic acid... s so that they can be visualized). They are safer because they are nonradioactive. 2. Name the three basic methods used to label a DNA probe:: 1. End Labeling 2. Nick Translation 3. Random priming 3. What is SDS? Name 3 of its applications in the lab.: Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used in SDS-PAGE to denature proteins so they separate by molecular weight. 3. Used in extractions to: A: Lyse cells (disrupt cell wall) B: Dissociates nucleic acid:protein complexes 4. Name the 10 subunits of Prokaryotic DNA Pol III Holoenzyme and their general function. Which subunits belong to the core enzyme and which belong to the camp loading complex?: ±²³´´'µ¸ÄÇÈ Alpha, Beta, Gamma, Delta, Delta prime, Epsilon, Theta, Tau, Chi, Psi Core Enzyme Complex: ±A-lpha: Has polymerase activity 5'>3'. µE-psilon: Has exonuclease activity 3'>5' (Proofreading). ¸-Theta: Stimulates exonuclease activity on µ. Clamp Loader Complex: ³-Gamma: Clamp loader (Binds ATP). ´-Delta: Clamp loader (Binds to ²subunit). ´'-Delta prime: Clamp loader (Binds to ´and ³). ÇC-hi: Clamp loader (Binds to SSB proteins). ÈP-si: Clamp loader (Binds to ´'and Ç). Other subunits: ²-Beta: Sliding clamp ÄT-au: Dimerizes the core (aka holds the core together) 5. In what phase is PCR product measured (analyzed) for standard PCR vs. qPCR (aka RT-PCR)?: Standard PCR = Stationary phase. (aka after the run is complete.) [Show More]

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