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Genetics Final Exam Questions and Correct Answers (Verified by Expert)

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Genetics Final Exam Questions and Correct Answers (Verified by Expert) 1 / 24 1. What is the function of single-strand-binding proteins? A. unwind the double helix by breaking hydrogen bonding between the two strands at the replication fork B. bind to oriC and cause a short section of DNA to unwind C. connect Okazaki fragments by sealing nicks in the sugar-phosphate backbone D. prevent the formation of secondary structures within single-stranded DNA E. reduce the torsional strain that builds up ahead of the replication fork as a result of winding: prevent the formation of secondary structures within single-stranded DNA 2. DNA polymerase I and DNA polymerase III both have activity but only DNA polymerase I has activity. A. 3'-5' exonuclease, 5'-3' exonuclease B. 5'-3' polymerase, 3'-5' exonuclease C. 3'-5' polymerase, 3'-5' polymerase D. 5'-3' polymerase, 3'-5' polymerase E. 5'-3' exonuclease, 3'-5' exonuclease: 32 - 52 exonuclease; 52 - 32 exonuclease 3. The Holliday model describes which of the following processes?: homologous recombination 4. What is the function of DNA gyrase? A. connects w okazaki fragments by sealing nicks in the sugar phosphate backbone B. prevents the formation of secondary structures within single stranded DNA C. binds to oriC and causes a short section of DNA to unwind D. unwinds the double helix by breaking the hydrogen bonding between the two strands at the replication fork E. reduces the torsional strain that builds up ahead of the replication fork as a result of unwinding: reduces the torsional strain that builds up ahead of the replication fork as a result of unwinding 5. The nuclear genome of a single human cell (i.e., the entire diploid complement) contains about 6.6 billion (6.6 × 109) base pairs of DNA. If synthesis at each replication fork moves along the template at an average rate of 50 nucleotides per second and all the DNA is replicated in 5 minutes, how many origins of replication exist in a human diploid cell? Assume that replicationGenetics Final Exam Questions and Correct Answers (Verified by Expert) 2 / 24 is initiated simultaneously at all origins. A. 888,000 B. 2.64 x 107 C. 1.32 x 108 D. 440,000 E. 220,000: 220,000 6. Approximately 6.6 Gb of DNA is present in a single-celled human zygote and must be replicated faithfully at each cell division. What is the approximate size of the haploid human genome? A. 1Mb B. 6.6 Gb C. 3.2 Gb D. 3.2 Mb E. 12.8 Gb: 3.2 Gb 7. You learn that a Mars lander has retrieved a bacterial sample from the polar ice caps.You obtain a sample of these bacteria and perform the same kind of experiment that Meselson and Stahl did to determine how the Mars bacteria replicate their DNA. Based on the following equilibrium density gradient centrifugation results, what type of replication would you propose for these new bacteria? (check image under question 7, take-home 9) A. semiconservative or dispersive B. semiconservative C. conservative or dispersive D. conservative E. dispersive: dispersive

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