TSI Tests 37 Questions with Verified Answers,100% CORRECT

TSI Tests 37 Questions with Verified Answers Red slant/ yellow butt - CORRECT ANSWER Glucose only. Protein used in slant Yellow slant/yellow butt - CORRECT ANSWER -Glucose + -Lactose and sucrose + Red slant/ re ...

TSI Tests 37 Questions with Verified Answers Red slant/ yellow butt - CORRECT ANSWER Glucose only. Protein used in slant Yellow slant/yellow butt - CORRECT ANSWER -Glucose + -Lactose and sucrose + Red slant/ red butt - CORRECT ANSWER -no fermentation Protein catabolized throughout Red slant only - CORRECT ANSWER Aerobic digestion of protein only No change in slant or butt - CORRECT ANSWER Slow or no growth Yellow slant/red butt - CORRECT ANSWER Not possible Black precipitate - CORRECT ANSWER Sulfur reduced Cracks in agar - CORRECT ANSWER Gas produced What does TSI mean? - CORRECT ANSWER Triple Sugar Iron What is a TSI (Triple Sugar Iron) Test also referred to as? - CORRECT ANSWER Kligers Iron Test What does a TSI Test, test for? - CORRECT ANSWER Bacteria's ability to: -Ferment glucose and lactose/sucrose -Produce gas -Reduce Sulfur What are the ingredients of this media? - CORRECT ANSWER Glucose, Sucrose, and Lactose What is the ratio of Glucose, Sucrose, and Lactose in this media? - CORRECT ANSWER 1:10:10 What is the sulfur source in a TSI Test? - CORRECT ANSWER Sodium Thiosulfate What is the fermentation indicator in a TSI Test? - CORRECT ANSWER Phenol Red What is the hydrogen sulfide indicator in a TSI Test? - CORRECT ANSWER Ferrous Sulfate What are the 2 steps to inoculating this media? - CORRECT ANSWER 1.) Stab the butt 2.) Fishtail the slant In the Fermentation of sugars portion of a TSI test, which sugar is limited, and why? - CORRECT ANSWER Glucose, because it is bacteria's primary source of carbon Which sugar will bacteria always try to ferment first? - CORRECT ANSWER Glucose If ONLY glucose is fermented, what color will the slant and butt be? - CORRECT ANSWER Red slant, Yellow butt If glucose is fermented, as well as lactose and/or sucrose, what color will the slant and butt be? - CORRECT ANSWER Yellow slant, Yellow butt If neither glucose nor sucrose/lactose is fermented, what color will the slant and butt be? - CORRECT ANSWER Red slant, Red butt In the Gas production portion of a TSI Test, it is only associated with ____ - CORRECT ANSWER Fermentation How is Gas production detected in a TSI Test? - CORRECT ANSWER By fissures in the medium, or lifting the agar In the Sulfur Reduction portion of a TSI Test, what is the reaction that occurs when sulfur is reduced? - CORRECT ANSWER Sodium thiosulfate --(reduction by bacteria)--> hydrogen sulfide --(+ ferrous sulfate (FeS))--> Black Precipitate Sulfur Reduction only occurs under - CORRECT ANSWER acidic conditions (so it is associated with fermentation) What does a black butt indicate in a TSI Test? - CORRECT ANSWER Sulfur reduction What does a red slant indicate in a TSI Test? - CORRECT ANSWER Only glucose is fermented What does a yellow slant indicate in a TSI Test? - CORRECT ANSWER Glucose AND/OR lactose and sucrose is fermented What would a (r/r), (-) gas, (-) sulfur result indicate? - CORRECT ANSWER Red slant, yellow butt, negative gas production, negative sulfur reduction What would a (r/y), (-) gas, (-) sulfur result indicate? - CORRECT ANSWER Red slant, yellow butt, negative gas production, negative sulfur reduction What would a (y/y), (-) gas, (-) sulfur result indicate? - CORRECT ANSWER Yellow slant, yellow butt, negative gas production, negative sulfur reduction What would a (y/y), (+) gas, (-) sulfur result indicate? - CORRECT ANSWER Yellow slant, yellow butt, positive gas production, negative sulfur reduction What would a (r/y), (-) gas, (+) sulfur result indicate? - CORRECT ANSWER Red slant, yellow butt, negative gas production, positive sulfur reduction What would a (y/y), (+) gas, (+) sulfur result indicate? - CORRECT ANSWER Yellow slant, yellow butt, positive gas production, positive sulfur reduction "If you have a yellow slant, yellow butt, no gas production, and no sulfur reduction, what happened?" - CORRECT ANSWER Glucose and/or sucrose and lactose were fermented Which organism is a sulfur reducer? - CORRECT ANSWER PROTEUS