Triple Sugar-Iron Agar Test Questions with Verified Answers,100% CORRECT

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Triple Sugar-Iron Agar Test Questions with Verified Answers Gram- - CORRECT ANSWER Is TSI gram+ or gram- used to differentiate members of Enterobacteriacea and to distinguish them from other gra ... m- rods - CORRECT ANSWER What is the application of TSI? 1 glucose, lactose & sucrose 2 animal protein 3 ferrous sulfate 4 sodium thiosulfate 5 phenol red - CORRECT ANSWER What is the media in TSI (triple sugar iron) carbohydrate for fermentation - CORRECT ANSWER What is the purpose of glucose, lactose & sucrose source of carbon & nitrogen - CORRECT ANSWER What is the purpose of animal proteins? source of oxidized sulfur & hydrogen sulfide indicator - CORRECT ANSWER What is the purpose of Ferrous sulfate? source of oxidized sulfur - CORRECT ANSWER What is the purpose of sodium thiosulfate? pH indicator - CORRECT ANSWER What is the purpose of phenol red? glucose & lactose and/or sucrose fermentation with acid accumulation in slant and butt - CORRECT ANSWER Yellow slant/ yellow butt glucose fermentation with acid production. Proteins catabolized aerobically (in the slant) with alkaline products (reversion) - CORRECT ANSWER Red slant/ yellow butt no fermentation. peptone catabolized aerobically and anaerobically with alkaline products. not from enterobacteriaceae - CORRECT ANSWER red slant/ red butt no fermentation. peptone catabolized aerobically with alkaline products. not from enterobacteriaceae - CORRECT ANSWER red slant/ no chang *********** organism is growing slowly or not at all. Not from enterobacteriaceae - CORRECT ANSWER no change in slant/ no change in butt sulfur reduction - CORRECT ANSWER black precipitate in the agar gas production - CORRECT ANSWER cracks in or lifting of agar the color of the slant determines which carbohydrates have been fermented - CORRECT ANSWER It the butt of an inoculated tube is totally black, what color do you assume the butt is? thiosulfate, the breakdown of cysteine in the peptone - CORRECT ANSWER Hydrogen sulfide may be produced by the reduction of ______ in the medium or by _______________. The microorgansim utilizes peptone aerobically & anaerobically and amino acids will alkalinize the medium and turn it red in the slant and butt. - CORRECT ANSWER It the medium has turned red, (slant and butt) what has happened? Enterobacteriaceae - CORRECT ANSWER TSI is used to primarily differentiate member of what family? enterobacteriaceae, bacilli - CORRECT ANSWER The triple sugar-iron agar test is used to distinguish between members of the genera _________, and between them and other groups of intestinal _______. carbohydrate fermentation, hydrogen sulfide - CORRECT ANSWER It is a screening procedure for ___________ and _____________ production 1, lactose, sucrose, 0.1, glucose - CORRECT ANSWER TSI agar slants contain __% _______ and _______ and ___% _________. phenol red - CORRECT ANSWER Acid production during carbohydrate fermentation can also be detected by the presence of the acid-base indicator _________. stab-and-streak, 18, 24 - CORRECT ANSWER determined by using a __________ procedure and is interpreted between ___ and ___ hours alkaline, red, acid, yellow, - CORRECT ANSWER ______ slant (_____) and ______ butt (____) with or without gas production (breaks ***********) = only glucose fermentation has occurred. glucose, acid - CORRECT ANSWER these organisms preferentially degrade ________ first, which is present in very low concentration and produces ______ oxidation, glucose, ammonia, peptones - CORRECT ANSWER The alkaline slant is the result of the rapid ________ of of the small amount of acid produced on the surface of the slant by the fermentation of _______ and the production of __________ by the degradation of ________. oxygen tension, growth - CORRECT ANSWER the acid butt is due to reduced oxidation of acids because of the lower __________ as well as slower _______ of the organism acid, yellow, yellow - CORRECT ANSWER ______ slant (________) and butt (_________) with or without gas production = lactose and/or sucrose have been fermented. The higher concentration of both of these sugars results in the continuous fermentation on the slant and *********** alkaline, red, peptones, ammonia, aerobic - CORRECT ANSWER _________ slant and butt (_____) = carbohydrate fermentation has not occurred. ________ are catabolized in place of carbohydrates and ________ is produced. It can also be determined whether the fermentation was aerobic or anaerobic. if only ________ catabolism occurred then only the slant will be alkaline. if aerobic and anaerobic has occurred then the slant and butt will be alkaline sodium thiosulfate, iron sulfate - CORRECT ANSWER the capability of certain microbes to produce gaseous hydrogen sulfide can be detected by the presence of __________. __________ is added to the medium to allow detection of the colorless gas black - CORRECT ANSWER the combination of hydrogen sulfide with ferrous sulfate produced iron sulfide, an insoluble _____ precipitate that colors the whole butt Triple Sugar Iron Test - CORRECT ANSWER What is the name of this test? Differentiate between gram-negative rods - CORRECT ANSWER What is the purpose of this test? Slant - Acid or Alkaline Butt - Acid or Alkaline Gas Production - + or - Hydrogen Sulfide Production - + or - - CORRECT ANSWER What is the order of the results of this test? Phenol Red - CORRECT ANSWER What is the pH indicator for carbohydrate fermentation? Ferrous Sulfate - CORRECT ANSWER What is the color indicator for hydrogen sulfide production? Sodium Thiosulfate - CORRECT ANSWER What is the substrate for hydrogen sulfide production? Glucose Lactose Sucrose Peptones - CORRECT ANSWER What are the substrates for carbohydrate fermentation? Yellow Tube 1 - CORRECT ANSWER What are the colors for an acidic reaction on the slant? Which tubes have an acidic reaction on the slant? Pink or Orange Tubes 2, 3 and 4 - CORRECT ANSWER What are the colors for an alkaline reaction on the slant? Which tubes have an alkaline slant? Black or Yellow Tubes 1, 2 and 4 - CORRECT ANSWER What are the colors for an acidic reaction on the butt? Which tubes have an acidic reaction on the butt? Pink or Orange Tube 3 - CORRECT ANSWER What are the colors for an alkaline reaction on the butt? Which tubes have an alkaline butt? (Slant) Acid / (Butt) Acid / (Gas) + / (H2S) + - CORRECT ANSWER In a TSI slant, if the slant has turned yellow and the butt has turned black with bubbles in the media, what is recorded? (Slant) Alk / (Butt) Acid / (Gas) - / (H2S) + - CORRECT ANSWER In a TSI slant, if the slant has turned pink and the butt has turned black, what is recorded? (Slant) Acid / (Butt) Acid / (Gas) + / (H2S) - - CORRECT ANSWER In a TSI slant, if the slant has turned yellow and the butt has turned yellow with bubbles in the media, what is recorded? (Slant) Alk / (Butt) Alk / (Gas) - / (H2S) - - CORRECT ANSWER In a TSI slant, if the slant has turned pink and the butt has turned pink, what is recorded? [Show More]

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