Biology > Lab Report > Georgia State University - BIO 3810Enzyme Kinetics Report (All)
Georgia State University - BIO 3810Enzyme Kinetics Report
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PART A: Standard Curve Tubes [Pyruvate] Average OD510 1 and 2 0 0.11 3 and 4 0.015 0.1595 5 and 6 0.03 0.278 7 and 8 0.06 0.006 9 and 10 0.09 0.634 11 and 12 0.12 0.721 PART B Sample Calc ... ulations y=5.0972X + 0.0505 Pyruvate concentration: 0.186 = 5.0972X +0.0505 X=(0.186-0.0505)/5.0972 =0.026 X=[Pyruvate]=0.026 mM Velocity V=(0.026 x 0.01)/6 = 4.33E-05 [ADP] CiVi =Cf Vf Cf = (15x0.25)/1.5 = 0.025 mM [Show More]
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