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QUESTIONS AND ANSWERS FOR BIOL 204 FINAL EXAM-ALL ANSWES CORRECT-GRADED A

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BIOL 204 – Biochemistry Name: SPRING 2017/REA/ELLIOTT/KARCH Penn ID (middle 8 digits): 1. The five proteins, listed in the ... table below with their estimated Mr and pI values, were separated by two-‐dimensional electrophoresis. Protein Mr pI a. Unknown 45,000 5.4 b. Cytochrome c 13,400 10.6 c. Myoglobin 17,000 7.0 d. Serum albumin 69,000 4.8 e. Transferrin 90,000 5.9 A. Indicate the likely positions of the proteins listed in the table on the Coomassie blue-‐stained gel shown below: 2.5 pts B. Assuming that all of the proteins in part A are monomeric and have similar hydrodynamic diameters, in what order would you expect them to elute from a gel-‐filtration column when it is run under non-‐denaturing conditions? 1 pt A. b, c, a, d, e B. e, d, a, c, b C. d, a, e, c, b D. b, c, a, e, d E. b, c, e, a, d a b c d e3 BIOL 204 – Biochemistry Name: SPRING 2017/REA/ELLIOTT/KARCH Penn ID (middle 8 digits): C. In what order would you expect the proteins in part A to desorb from a strong cation-‐exchange resin eluted with a linear gradient of NaCl? 2.5 pts A. b, c, a, d, e B. e, d, a, c, b C. d, a, e, c, b D. b, c, a, e, d E. b, c, e, a, d 2. [Show More]

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