Pearson Edexcel Level 3 GCE,./ Biology B Advanced PAPER 1: Advanced Biochemistry, Microbiology and Genetics. 99% proven pass rate.

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Answer ALL questions. Some questions must be answered with a cross in a box . If you change your mind about an answer, put a line through the box and then mark your new answer with a cross . 1 The ... polymerase chain reaction (PCR) and gel electrophoresis are used to produce DNA profiles. The diagram shows DNA profiles, from an adult female, an adult male and each of four children. Adult female Adult male Child 1 Child 2 Child 3 Child 4 (a) Explain why PCR is used before gel electrophoresis to produce these DNA profiles. (2) .................................................................................................................................................(b) Explain why gel electrophoresis produces individual DNA profiles. (2) ..................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................................................................... ..................................................................................................................................................................................................................................................................................... (c) Which of the children have both of these adults as their parents? (1) A child 1 and child 2 B child 1 and child 3 C child 2 and child 3 D child 2 and child 4 (Total for Question 1 = 5 marks)4 *P65455A0432* 2 An investigation was carried out to compare the aerobic respiration of different sugars by yeast. A suspension of yeast was used to produce three cultures, each one with a different sugar. A control was set up that had a suspension of yeast cultured with water only. The volume of carbon dioxide produced was measured. The mass of yeast and the concentration of each sugar were controlled. The graph shows the results of this investigation. Volume of carbon dioxide produced / cm3 0 10 20 30 40 50 60 25 20 15 10 5 0 Time / mins maltose glucose water lactose (a) (i) Give one difference between the structure of glucose and the structure of maltose. (1)...................................................................................................................... [Show More]

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