Q 3 / 3 pts uestion 1
Your Answer:
The word ‘antigen’ is actually a combination for what two words?
Antigen is the combination of antibody generating pathogen – something in system going to require
antibiotics to fig
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Q 3 / 3 pts uestion 1
Your Answer:
The word ‘antigen’ is actually a combination for what two words?
Antigen is the combination of antibody generating pathogen – something in system going to require
antibiotics to fight it
Antibody-generating
Q 3 / 3 pts uestion 2
Your Answer:
For the ELISA assay depicted below, what do the arrows indicate?
The arrows indicate the wash steps.
The arrows indicate the wash steps.
Q 4 / 4 pts uestion 3
Your Answer:
What would happen in terms of the colorimetric readout if you forgot to do the wash step after Step D
This study source was downloaded by 100000830772748 from CourseHero.com on 07-05-2022 05:58:45 GMT -05:00 (above)?
https://www.coursehero.com/file/131793702/Lab-Exam-8pdf/
2/22/22, 3:44 PM L8: Lab Exam 8: Essential Microbiology with Lab- Kephart
https://nursingabc.instructure.com/courses/135/quizzes/5845?module_item_id=16383 2/5
All the wells would be dark in color
All of the wells would have a dark color. The intensity of the readout is dependent upon the
amount of the labeled secondary antibody present in the well. Since the unbound secondary
antibody was not washed away, all wells would react equally.
Ques 2.5 / 2.5 pts tion 4
Your Answer:
In a western blot ____________ are separated based on its size.
Proteins
proteins
Ques 2.5 / 2.5 pts tion 5
Your Answer:
When running a western blot, what is the purpose of the marker?
The purpose of the marker is to provide a point of reference to the size of proteins
The marker serves as molecular weight standards for the assay. The marker contains a
collection of known molecular weight bands (also known as a molecular weight ladder) to be
used in comparison to the samples being run on the gel.
Ques 0 / 2.5 pts tion 6
Your Answer:
When developing a western blot what two factors influence the intensity of the band?
Two factors that influence the intensity of the band are the type of membrane used and the intensity of the
set current
Both the amount of protein loaded as well as the duration (time) of development is directly
proportional to band intensity.
Ques 2.5 / 2.5 pts tion 7
This study source was downloaded by 100000830772748 from CourseHero.com on 07-05-2022 05:58:45 GMT -05:00
https://www.coursehero.com/file/131793702/Lab-Exam-8pdf/
2/22/22, 3:44 PM L8: Lab Exam 8: Essential Microbiology with Lab- Kephart
https://nursingabc.instructure.com/courses/135/quizzes/5845?module_item_id=16383 3/5
Your Answer:
Identify (specific name) given to the region of the western blot indicated by the arrow.
Dye front
The arrow is pointing to the dye front.
Ques 2 / 2 pts tion 8
Your Answer:
What is the common name for agglutination?
Clumping
Clumping
Ques 2 / 2 pts tion 9
In the wet lab portion of the video, what pathogen was the agglutination assay specifically designed to test
for? Choose all that apply.
E coli Salmonella
Staph Correct!
Strep
Ques 2 / 2 pts t
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