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BIO 171

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Title: Introduction of Medical Microbiology Objective: Cultivation of samples (growth conditions) -Equipment used Identification of samples (biochemical assays) ex) what kind of bacteria -Test av ... ailable Evaluation of samples (microscopy) -Visualization and recognition of key characteristics Procedure: Basic equipment; 1. Cleaning; Autoclave 125 °C; uses heat, pressure, and steam to sterilize your environment and tools. This means all bacteria, viruses, fungi and spores are inactivated by Autoclave. 2. Growing; fixed incubator 37 °C Shaker incubator 37 °C 3. Visualizing; Microscopy 4. Storing; refrigerator 4°C – slows the bacteria growth and helps preserve sample. General Lab Safety 1. Never eat or drink in the lab a. Contamination risks 2. Use personal protective equipment (PPE) a.Latex or thermal gloves (application dependent) b.Eyewear protection c.Lab coat 3. Never leave the lab while wearing PPE a.Public hallway b.Bathroom c. Cafeteria Lab notebook: First Name initial, last name initial Exp# Title of Experiment PL01 Keeping a lab notebook Objective; to establish an organized template for keeping experimental records procedures and results. Procedure; 1. This is where each step of a given protocol is recorded 2. Be sure to clearly indicate any deviations that may occur during the experiment. Notes; Additional (helpful) information placed here Results; a summary of the final outcome of the experiment should be described here. Notes: If I have instructor questions, I can send messages to my instructor using the Canvas Messages [Show More]

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