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Separation of Organelles / Fractionation of Cells - ANSWER Differential Centrifugation 1. Tissue Homogenization 2. Low speed centrifugation (whole cells, nuclei, membrane) 3. Medium speed centrifugation (mitochondria ...

Separation of Organelles / Fractionation of Cells - ANSWER Differential Centrifugation 1. Tissue Homogenization 2. Low speed centrifugation (whole cells, nuclei, membrane) 3. Medium speed centrifugation (mitochondria, lysosomes, peroxisomes) 4. High speed centrifugation (microsomes -fragments of ER) 5. Very high-speed centrifugation (soluble proteins) Isopycnic Centrifugation - ANSWER Sucrose-Density (components separated by density) Interactions occur... - ANSWER because of the electrostatic properties of molecules. - these properties can involve full, partial or momentary charges Examples of electrostatic interactions - ANSWER dipole-dipole interactions hydrogen bonds ionic interactions van der Waals interactions Hydrophobic Interactions... - ANSWER entropy, not an attraction between hydrophobic molecules The special properties of water... - ANSWER derived from its polarity and hydrogen bonding - overall polarity is due to polar bonds and shape Entropy & 2 Critical Processes that involve Water - ANSWER 1. Dissolving of highly ordered solids (salts) - entropy of some of the water is decreased, but the entropy of the ions is greatly increased 2. The hydrophobic effect - there is no strong attraction between hydrophobic molecules (van der Waal's only) - there is no repulsion between hydrophobic molecules and water Aggregation of H-bonds in water is driven by - ANSWER entropy change of water (decrease) - it can also drive other reactions such as enzyme-substrate interaction Ion Product Constant - ANSWER [H+][OH-] = Keq [HOH] = 1.0 x 10^-14 Equilibrium Constant - ANSWER Keq = [H+][OH-]/[HOH] 2 Applications of pH - ANSWER 1. Controlling pH with buffers 2. How pH (protonation) affects weak interactions of molecules (charge and ability to form H-bonds) pKa = - ANSWER -log Ka pKa definition - ANSWER pH at which half of the acid is ionized. titration curves reveal the pKa of weak acids - is a measure of affinity for protons a high pKa constitutes... - ANSWER it takes a high pH (lots of OH-) to remove the H+ from the acid - the acid has a high affinity for H+ - if the pKa is high, it has a HIGH affinity (want) to grab onto H+, because of the large amount of OH- Significance of Buffering - ANSWER 1. Homeostasis 2. This phenomenon is very important for biochemical activity - many reactions depend on the state of protonation of particular functional groups A buffer is made... - ANSWER from a weak acid and its conjugate base Henderson-Hasselbalch Equation - ANSWER pH = pKa + log ([A-]/[HA])

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Biochemistry ACS Final Exam latest updated 2022 ACS BIOCHEMISTRY EXAM – OBU Latest Graded A+ ACS BIOCHEMISTRY EXAM Q&A With complete solution

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