BioChemistry > EXAM > Biology and Biochemistry 1 University of Houston FlyLab JS Simulations and Chi-square analysis (50 (All)

Biology and Biochemistry 1 University of Houston FlyLab JS Simulations and Chi-square analysis (50 pts) Lab Activity 1 – Part 1:

Document Content and Description Below

Lab Activity 1 – Part 1: FlyLab JS Simulations and Chi-square analysis (50 pts) List the names of the students you are working with: 1. Design a wild-type female fly and cross it to a male fly with curved wings. a. What is the phenotype(s) of the offspring? b. Perform an F2 cross using the progeny from the F1 cross. What do the offspring look like? Phenotype # of Males # of Females c. Perform the reciprocal cross. What is the appearance of the F1 and F2 offspring? d. Create a hypothesis of the mode of inheritance for curved wings. i. Null hypothesis: ii. Alternative hypothesis: Copyright Dr. Marianne Kramer, University of Houston, Department of Biology and Biochemistry 2 e. Perform a Chi-square analysis to determine the validity of your null hypothesis. Phenotype Observed Expected O-E (O-E)2 (O-E)2/E X2: i. What is your Chi-square value? ii. Do you reject of fail to reject the null hypothesis? Explain. iii. Write a 1-2 sentence conclusion about the gene causing curved wing phenotype. f. Draw the Punnett square for the F1 and F2 cross: F1 Male: Female: F2 Male: Female: Copyright Dr. Marianne Kramer, University of Houston, Department of Biology and Biochemistry 3 2. Design a wild-type female fly and cross it to a male fly with forked bristles. a. What is the phenotype(s) of the offspring? b. Perform an F2 cross using the progeny from the F1 cross. What do the offspring look like? Phenotype # of Males # of Females c. Perform the reciprocal cross. What is the appearance of the F1 offspring? F2 offspring? d. Create a hypothesis of the mode of inheritance for curved wings. i. Null hypothesis: ii. Alternative hypothesis: e. Perform a Chi-square analysis to determine the validity of your null hypothesis. Phenotype Observed Expected O-E (O-E)2 (O-E)2/E X2: i. What is your Chi-square value? Copyright Dr. Marianne Kramer, University of Houston, Department of Biology and Biochemistry 4 ii. Do you reject of fail to reject the null hypothesis? Explain. iii. Write a 1-2 sentence conclusion about the gene causing forked bristle phenotype. f. Draw the Punnett square for the F1 and F2 cross: F1 Male: Female: F2 Male: Female: Copyright Dr. Marianne Kramer, University of Houston, Department of Biology and Biochemistry 5 3. Design a wild-type female fly and cross it to a male fly with lobe eyes. a. What is the phenotype(s) of the offspring? b. Perform an F2 cross using the progeny from the F1 cross. What do the offspring look like? Phenotype # of Males # of Females c. Perform the reciprocal cross. What is the appearance of the F1 offspring? F2 offspring? d. Create a hypothesis of the mode of inheritance for lobe eyes. i. Null hypothesis: ii. Alternative hypothesis: e. Perform a Chi-square analysis to determine the validity of your null hypothesis. Phenotype Observed Expected O-E (O-E)2 (O-E)2/E X2: i. What is your Chi-square value? Copyright Dr. Marianne Kramer, University of Houston, Department of Biology and Biochemistry 6 ii. Do you reject of fail to reject the null hypothesis? Explain. iii. Write a 1-2 sentence conclusion about the gene causing lobe eyed

[Show More]

Last updated: 1 month ago

Preview 4 out of 13 pages

Get Access

Instant download after payment

Card Payments

₿ Crypto Accepted

Instant download