What's the overall purpose of this virtual lab? - ANS -Familiarize with science and techniques used to identify different types of bacteria based on their DNA sequence
What are the four basic steps involved in this ba
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What's the overall purpose of this virtual lab? - ANS -Familiarize with science and techniques used to identify different types of bacteria based on their DNA sequence
What are the four basic steps involved in this bacterial identification lab? - ANS -1. Prepare sample from patient and isolate whole bacterial DNA
2. Make many copies of desired piece of DNA
3. Sequence the DNA
4. Analyze the sequence and identify the bacteria
What is 16s rDNA and how is it used to identify species of bacteria? - ANS -16s rDNA is a piece of DNA used for identifying bacteria the region codes for a small subunit of ribosomal RNA.
Identification relies on matching the sequence form your sample against a database of all known 16s rDNA sequences
As the pathology lab technician what's your task in this virtual lab? - ANS -Identify a bacterial sample received from a clinician
Extracting DNA involves which initial step? - ANS -Dissolving the cell wall with a digestive buffer (in white capped bottle)
What is the wire ring used for? - ANS -Obtain a sample form the Petri dish (extracts a colony)
Why are the proteolytic enzymes necessary? - ANS -Buffer is a digestive enzyme that east the cell wall in order to extract the bacterial DNA
Why do you need to inactivate the proteolytic enzymes and how do you do it? - ANS -Other enzymes are used in next step and enzyme is denatured by heating sample in a H2O bath @ 100 °C
After removing the enzymes why do you spin the sample in a centrifuge? - ANS -So cellular debris is spun down in the centrifuge and will appear as a solid deposit (pellet) @ bottom of the tube
What is the pellet? - ANS -Solid debris
What is supernatant? - ANS -clear liquid found on top of solid solution after a mixture has been centiruged contains the inclusion bodies
Where is the DNA? - ANS -Contained in the supernatant (liquid) in the PCR tube
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