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GENERAL BIOCHEMISTRY 115:404/504 | FOR QUALITY GRADES

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Part I. Answer all questions on the answer sheet. Give the answer which is most nearly correct. Unless otherwise indicated each question has only one answer. Each question is worth two points. There is information on ...

Part I. Answer all questions on the answer sheet. Give the answer which is most nearly correct. Unless otherwise indicated each question has only one answer. Each question is worth two points. There is information on the last page of this exam that may be useful in answering some quëstions. 1. Substrate level phosphorylation: A. describes the conversion of ADP into ATP with the addition of inorganic phosphate every place throughout the cell. B. describes the formation of ADP by phosphoryl group transfer from 1,3 bisphosphoglycerate. C. is the formation of ATP by phosphoryl group transfer from a higher energy compound. D. requires an enzyme that is catalyically perfect to function efficiently E. is always reversible under the conditions operating within the cell 2. Mutases are: A. enzymes that regulate glycolysis by changing the coding sequence of DNA B. proteins that regulate glycolysis by binding to glycolytic enzymes and mutating their function C. isomerases that catalyze the transfer of phosphoryl groups from one part of a substrate molecule to another. D. Isomerases that catalyze the transfer of phosphoryl groups from one molecule to another, “mutating” it. E. It will fold into a beta-pleated sheet. 3. The biosynthesis of lactose (-D-galactopyranosyl (14) – -D-glucopyranose) couples which two monomers? A. UDP-Glucose and Galactose B. UDP-Glucose and UDP-Galactose C. UDP-Galactose and Glucose D. Glucose and Galactose E. N-acetyl galactosamine and Glucose 4. The Entner-Doudoroff pathway evolved earlier in evolution than the EMP pathway. One difference between the two pathways is: A. The ED pathway does not generate NADH reducing equivalents. B. The ED pathway results in a net of 3 ATP molecules per molecule of glucose while the EMP pathway results in a net of 4 ATP per molecule of glucose C. The ED pathway is used by organisms that perform gluconeogenesis but lack phosphofructokinase-1 D. The ED pathway is the exact reverse of gluconeogenesis but the EMP pathway is regulated with bypass reactions. E. The reducing equivalents of the ED pathway are

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