Using the observations made and recorded from PL09 Unknown Pathogen,
complete the results sections accordingly.
Given Culture A and Culture B were accidentally mixed, what observations were made
from the Gram stain (s
...
Using the observations made and recorded from PL09 Unknown Pathogen,
complete the results sections accordingly.
Given Culture A and Culture B were accidentally mixed, what observations were made
from the Gram stain (shown below)?
Your Answer:
Gram-positive cocci (spherical) clusters and Gram-negative bacillus (rods) were
observed.
Gram-positive cocci (spherical) clusters and Gram-negative bacillus (rods) were
observed.
Question 2
Not yet graded / 6 pts
Using the observations made and recorded from PL09 Unknown Pathogen,
complete the results sections accordingly.
Given the results from a MacConkey agar plate shown below, what is the shape of
Culture A? Culture B?
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Your Answer:
MacConkey agar is selective for Gram-negative bacteria. Thus, Culture A must be Gram
positive as no growth was observed. Coupled to the results of the gram stain, The
Culture A (Gram-positive) must be the cocci. Culture B must be Gram-negative (growth
observed) and this correlates to the bacillus or rod-shaped bacteria.
MacConkey agar is selective for Gram-negative bacteria. Thus, Culture A must be
Gram-positive as no growth was observed. Coupled to the results of the gram
stain, Culture A (Gram-positive) must be the cocci.
Culture B must be Gram-negative (growth observed) and this correlates to the
bacillus or rod-shaped bacteria.
Question 3
Not yet graded / 6 pts
Using the observations made and recorded from PL09 Unknown Pathogen,
complete the results sections accordingly.
The hemolytic properties of both cultures were next examined by streaking each culture
on a blood agar plate (shown below). What is the hemolytic status of Culture A? Culture
B?
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Your Answer:
Culture A exhibits beta hemolysis as defined by the region of clearing around the
growing bacterial streak.
Culture B exhibits gamma-hemolysis (non-hemolytic) as indicated by the white colonies
growing on the still red agar.
Using the observations made and recorded from PL09 Unknown Pathogen, complete
the results sections accordingly.
Culture A exhibits beta hemolysis as defined by the region of clearing around the
growing bacterial streak.
Culture B exhibits gamma-hemolysis (non-hemolytic) as indicated by the white
colonies growing on the still red agar.
Question 4
Not yet graded / 6 pts
Using the observations made and recorded from PL09 Unknown Pathogen,
complete the results sections accordingly.
Culture A and Culture B were assessed via the catalase test as shown below. State your
observations for each culture.
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https://www.coursehero.com/file/94661412/BIOD-171-lab-9-examdocx/
Your Answer:
Culture A and B were both catalase positive meaning both are able to breakdown
hydrogen peroxide (damages cellular integrity) into harmless hydrogen and water
molecules.
Culture A and B were both catalase positive meaning both are able to breakdown
hydrogen peroxide (damages cellular integrity) into harmless hydrogen and water
molecules.
Question 5
Not yet graded / 6 pts
Using the observations made and recorded from PL09 Unknown Pathogen,
complete the results sections accordingly.
Culture A and Culture B were assessed via the oxidase test as shown below. State y
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