Frieda Carson Workbook - Processing (2) with complete solution

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Frieda Carson Workbook - Processing (2) with complete solution 1 / 10 1. Which of the following is a dehydrating agent? a) Formalin b) Xylene c) Benzene d) Alcohol: Alcohol 2. A clearing agent for use in processing tissues for paraffin embedding must be miscible with the: a) Fixative and paraffin b) Dehydrant and paraffin c) Fixative and dehydrant d) Paraffin and water: Dehydrant and Paraffin 3. Dioxane is a reagent that can be used: a) For both fixing and dehydrating tissues b) For both dehydrating and clearing tissues c) In very small volume ratios d) For long periods without changing: For both dehydrating and clearing tissues 4. A reagent that CANNOT be used for dehydrating tissue is: a) Benzene b) Absolute alcohol c) Dioxane d) Acetone: Benzene 5. Most commonly, the paraffin used for embedding tissues is kept at approximately: a) 37C b) 52C c) 60C d) 65C: 60C 6. A disadvantage of the use of dioxane in processing tissues is that it is: a) Toxic b) Likely to harden tissue c) Not miscible with water d) Explosive: Toxic 7. The chief objection to the use of Xylene as a clearing agent for processing tissues is that xylene is: a) Likely to harden tissue b) HydroscopicFrieda Carson Workbook - Processing (2) with complete solution 2 / 10 c) Only slightly miscible with paraffin d) The most volatile of the hydrocarbons: Likely to harden tissue 8. A good paraffin for routine use is one with a melting point of: a) 50C to 52C b) 55C to 58C c) 60C to 62C d) 63C to 65C: 55C to 58C 9. Which of the following chemicals is NOT a clearing agent? a) Chloroform b) Dioxane c) Ethanol d) Xylene: Ethanol 10. Overheating of the paraffin used to embed may cause: a) Air bubbles in the block b) Cracking of the block c) Distortion of the tissue cells d) Poor infiltration: Cracking of the block 11. One advantage of the paraffin technique is that: a) Hard objects cut easily b) There is practically no tissue distortion c) Serial sections are easy to obtain d) Microtomy artifacts are uncommon: Serial sections are easy to obtain 12. When cutting paraffin embedded tissues, if the tissues seems hard and brittle, one source of trouble is likely to be: a) Inadequate fixation b) Incomplete dehydration c) Overheated paraffin d) Poor infiltration: Overheated paraffin 13. The process of removing water from tissue is called: a) Dehydration b) Reduction c) Oxidation d Clearing: Dehydration

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