BIOCHEM C785 2ndOA (1)/ BIOCHEM C785 Readiness Questions with answers/BIOCHEM C785 BIO notes latest 2022/ BIOCHEM C785 2 OA readiness check complete/ Biochemistry Readiness Check II (Download to score an A)

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BIOCHEM C785 2ndOA (1)/ BIOCHEM C785 Readiness Questions with answers/BIOCHEM C785 BIO notes latest 2022/ BIOCHEM C785 2 OA readiness check complete/ Biochemistry Readiness Check II (Download to sc... ore an A) The 2nd OA Readiness Check is designed to give you additional practice with new assessment-style questions to help prepare for success on the OA. Thus, please try to mimic exam-like conditions by taking the test in one <2 hr sitting without any study aids apart from your whiteboard/scrap paper. Note: You can take the 2nd OA Readiness Check again if you're so inclined, but you can not edit your response as you may have done with the topic questions. You will be able to see the results afterwards. 1. What is the complementary sequence to 3’ ATG CGA ATA 5’? * 1/1 3’ TAT TCG CAT 5’ 3’ TAC GCT TAT 5’ 3’ GAT AGC ATA 5’ 5’ ATA AGC GTA 3’ 2. Which amino acid sequence would result from this Coding DNA strand? 3’ ATG CAG ATA 5’? * 1/1 Feedback The correct answer is 3' TAT TCG CAT 5'. Remember complementary means “Matching or Pairing” You have to remember to pay attention to your numbers as well as your letters (A-T, G-C, 5'-3'). The correct answer is 3’ ATG CGA ATA 5’ (original sequence) 5’ TAC GCT TAT 3’ (complementary sequence) But we asked for it in the 3’ direction, so mirror answer to give correct answer: 3’ TAT TCG CAT 5’Tyr Val Tyr Ile Gln Ile Ile Asp Val Leu His Lys 3. Which amino acid sequence would be made from this template DNA strand? 5’ TAT TAC CGA 3’? * 0/1 Feedback The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are non-complementary and parallel. So we copy the coding strand , change T ---> U, and then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’ mRNA Mirror by changing orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val (chart is in direction of 5' ---> 3')Tyr Tyr Arg Ile Met Ala Ser Val Ile Ser His Gln 4. Which of the following are the correct components for a PCR reaction? * 1/1 dNTPs, Primer, RNA Polymerase, template RNA dNTPs, Primer, DNA Polymerase, template DNA ATP, Primer, mRNA polymerase; template mRNA Correct answer Ser Val Ile Feedback The correct answer is C because 5’ TAT TAC CGA 3’ template is complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val IleAcetyl CoA, RNA primer, DNA Ligase, Template phosphate 5. If you had a mother who is homozygous for a recessive disease, and a father who is a heterozygous carrier, what percentage of children will have the disease? * 1/1 0% 25% 50% 75% 100% 6. Which of the following would represent a silent mutation if this is the original sequence: 5’ AUC GUA ACA 3’? * 1/1 Feedback The correct answer is c 50% Homozygous recessive: aa Heterozygous: Aa Feedback The correct answer is b5’ AUC GGA ACA 3’ 5’ AUA GUA ACA 3’ 5’ AUC GUA UAA 3’ 5’ AUC CUC ACA 3’ 7. If the original coding sequence is 5’ CGA TAC TTC AGA 3’ and it is mutated to 5' CGA TAT TTC AGA 3', what type of mutation would have taken place? * 1/1 Feedback The correct answer is B AUC GUA ACA Ile Val Thr Ile Gly Thr Missense Ile Val Thr Ile Val STOP nonsense Ile Leu Thr Missense [Show More]

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