A-Level Biology – OCR
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What are the pros and cons of an electron microscope? - Pros: Very high magnification and excellent resolution. Cons: specimen has to be dead, very expensive, very large, needs great skill and trainin... g to use. What is the difference between a transmission and an scanning electron microscope? - TEM sends a beam of electrons through the specimen, the SEM bounces electrons off the surface. What is the difference between light and electron microscopes? - Light uses lenses to focus a beam of light. Electron uses a beam of electrons, focused by magnets. What is an eye piece graticule? - A small ruler fitted to a light microscope's eyepiece. It must be calibrated using a stage micrometer before being used to measure specimens. What is a stage micrometer? - A millimeter long ruler etched onto a slide. it has 100 divisions, each of 0.01mm or 10 micrometers. It is used to calibrate the eyepiece graticule Why do we stain specimens? - To provide more contrast, and make it easier to distinguish certain parts. [Show More]
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