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University of Texas, KEY BIO 325 Genetics Third exam. A+ GRADED

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KEY BIO 325 Genetics Third exam. Name_____________________¬¬¬¬¬____________ UTEID________________________________ Read each question very carefully and make sure you are answering t... he question asked. Please make your answers are extremely clear and as short as possible. Place a circle or box around your final answer. You will not get credit if nothing is circled or boxed. Circle EVERY answer, not just the multiple guess question. You may use a calculator if necessary. If you want to have the possibility of a regrade, write your answers in pen. The test is over at 11:15. GOOD LUCK! For the short answer questions, PLEASE MAKE YOUR ANSWER SHORT. 1. A piece of DNA was sequenced by the Sanger dideoxy method, using radioactively labeled primers to allow visualization of the fragments produced. The autoradiogram of the electrophoretic results is shown below. Determine the sequence and polarity of the DNA (newly synthesized) strand. 8 pts Because DNA is synthesized 5’ to 3’, the shortest fragment is most 5’ of the new strand and longest is most 3’. 5'-CGCATTTGGG-3' Now design a 5 base pair primer that that you could use to continue to sequence along the template beyond the 3’ end of this new piece of DNA. Show the sequence and polarity. 6 pts (3 for sequence, 2 for orientation (gave pts for orientation even if sequence was not right)) Any primer that matches part of the sequence above can be used to continue sequencing along the template DNA beyond the 3’ end of this piece of DNA. Examples 5'-TTGGG-3'or 5'-ATTTG-3' etc. 2. Translate the following very short mRNA. 4 pt total 5'GMETH-UUG AUG CCU GAA CCC GGG UUU AAA UGA GCUUUUAAG AAAAAAAAA- 3' met pro glu pro gly phe lys stop 2 pt. What kind of organism is it from? How do you know? Eukaryote 2 pt 5’ methyl cap; Poly A tail 2pt 22 points possible You want to amplify the coding region from the start to stop codons from a cDNA of this mRNA but no other sequence. Design 5 base pair primers to do this. Show the sequence and polarity. 8 points 5’AUGCC TTACT5’ or 5’TCATT [Show More]

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