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ACD/CPD/CPD2 - ✔✔21 days CPDA-1 - ✔✔35 days Infants HDFN from ABO - ✔✔Spherocytes 1st pregnancy O mother Permanent deferral - ✔✔Hepatitis after 11 HIV T. Cruzi CJD 1 unit or ... PRBC - ✔✔Raises hemoglobin 1g and hematocrit 3% Leukoreduced RBCs - ✔✔Less than 5 x 10^6 to prevent febrile non hemolytic reactions Cryoprecipitate - ✔✔Factor 8 vWFFibrinogen For DIC 6 hours Platelets 1unit - ✔✔5000 10000 Irradiated blood - ✔✔Prevent GVHD Ulex europaeus - ✔✔Anti H lectin Dolichos biflorus - ✔✔Anti A1 lectin Amount of H greatest to least - ✔✔O A2 B A1 A1B Most immunogenicity antigen - ✔✔D Weak D - ✔✔Negative immediate spin and positive AHGWeak D donor - ✔✔Must be labeled Rh positive Weak D patient - ✔✔Receives D negative D control - ✔✔AB positive Any D negative IgM antibodies - ✔✔I H MN P1 Lewis IgG antibodies - ✔✔Rh group K Duffy Jk I, i antibody - ✔✔i converts to I as infant matures Destroyed by enzymes - ✔✔MN SDuffy Enhanced by enzymes - ✔✔Rh Kids Lewis I P1 Dosage - ✔✔MNS Rh Kids Duffy Top three acute/immediate transfusion reactions and mortality - ✔✔TRALI, hemolytic transfusion reactions and TACO IgE antibodies - ✔✔Mild Allergic reactions IgA antibodies - ✔✔Severe transfusion allergic reaction Anaphylactic Positive hemolysis with negative DAT - ✔✔Sickle cell crisisThalassemia/G6PD deficient Unit overheated or frozen All cells hemolysis Kernicterus - ✔✔Excess bilirubin in newborn HDFN from Rh - ✔✔Retics DAT positive Immediate jaundice After first baby Rosette - ✔✔Screening for fetal hemorrhage Kleihauer Betke - ✔✔Quantitative fetal maternal bleed Primary immune response - ✔✔IgM Secondary immune response - ✔✔IgG Type 1 hypersensitivity - ✔✔Anaphylactic ImmediateType 2 hypersensitivity - ✔✔Antibody depending cytotoxicity Transfusion reactions Hashimotos Good pasture Type 3 hypersensitivity - ✔✔Immune complex Rheumatoid arthritis SLE Type 4 hyper sensitivity - ✔✔Delayed Monocytes and lymphocytes Ouchterlony - ✔✔Antibodies added to pre-cut wells in center of agar plate and patient Sera and standards are alternated in wells surrounding the center well EIA/ELISA - ✔✔Sandwich technique HCG Nephelometry - ✔✔Insoluble complexes Why is pass-through suspension scattered light absorbance is proportional to the number of insoluble complexes compared to standards Antibody concentrationImmunofluorescence direct - ✔✔Add florescence labeled anti-body to patient tissue wash and examined under fluorescent microscope Immunofluorescence indirect - ✔✔Add patient serum to reagent wash add florescence label to anti-globulin wash and examined under microscope FPIA (Fluorescence Polarization Immunoassay) - ✔✔Add reagent antibody and fluorescent tact antigen to patient serum Increase polarize light as a negative test decrease polarized light as a positive test Sensitivity - ✔✔TP/ TP + FN x 100 Specificity - ✔✔TN / TN + FP x 100 Non lattice - ✔✔More sensitive immunoassays nephelometry Lattice - ✔✔Less sensitive C reactive protein - ✔✔Acute phase protein InflammationSyphilis - ✔✔T palladium FTA abs TPI Dark field microscopy VDRL - ✔✔Syphilis CSF screening but can be positive for malaria RPR - ✔✔Charcoal for syphilis More sensitive but Les specific infectious mononucleosis - ✔✔EBV Lymphocytes B cells Burkets disease? Autoimmune diseases - ✔✔SLE Sjögren's syndrome Scleroderma RA Recent acute hepatitis A infection - ✔✔Anti HaVAcute hep B infection - ✔✔Highly infectious HBsAg HBeAg Chronic hep B - ✔✔Carrier Anti HBc Past infection immunity to hep B - ✔✔Anti HBe Anti HBc Anti HBs Vaccine immunity to hep B - ✔✔Anti HBs Hep c infection - ✔✔Anti HcV Graves' disease - ✔✔Hyperthyroidism Low TSH Increase production T3 and T4 Weight loss and anxiety Hadimotos - ✔✔Hypothyroidism Increase TSHWeight gain lethargy intolerance to cold Thyroglobulins rheumatoid arthritis - ✔✔IgG Fc RA factor CA 125 - ✔✔Ovarian cancer CA 19-9 - ✔✔Pancreases cancer CEA - ✔✔Colon breast lung cancer CA 15-1 - ✔✔Breast cancer Transferrin - ✔✔Iron transport Ferritin - ✔✔Iron storage Hemoglobin F - ✔✔Alpha and gamma globlin chains Hemoglobin a - ✔✔Alpha and beta globin chainsLeft shift - ✔✔Decreased CO2, increased pH with high affinity and decreased 23DPG Right shift - ✔✔Increased CO2 Decreased pH Increased two, three DPG Hemoglobin Reference range - ✔✔Males 14-18 Females 12-16 Hematocrit reference ranges - ✔✔Males is 42% to53% Females are 38% of 47% MCV - ✔✔800-100 Hct/rbc x 10 MCH - ✔✔28-32 pg Hgb/rbc x 10 MCHC - ✔✔32-36% Hgb/ hct x 100Rule of 3 - ✔✔Hgb x3 = hct RDW - ✔✔11-14% WBC reference range - ✔✔5000-10000 Neutrophils reference range - ✔✔Relative 45-70% Bacteria RBC RR - ✔✔Males 4.5-6.1 x 10^6 Females 3.8-5.2 x 10^6 Lymphocyte RR - ✔✔Relative 20-40% Viral infection Monocyte - ✔✔Relative 3-10% Basophils - ✔✔Relative 0-2% Inflammation response mediator Eosinophils - ✔✔Relative 0-3%Allergic response Hyper-segmented neutrophils - ✔✔Megaloblastic anemia B12 and folate deficiency BAnd neutrophils (hypo-segmented) - ✔✔PelegerHuey Aml Aids Uncleared red cell WBC correction - ✔✔Wbc X 100 / 100+NRBC Dohle bodies - ✔✔May hagglin Hazy blue inclusion on NEUTROPHILS May hagglin - ✔✔Dohle bodies Thrombocytopenia with stress platelets Myeloproliferation Chediak higashi disease - ✔✔Giant lysosomal granules in granulocytes monocytes and lymphocytes Infections and bleeding [Show More]

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