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THE EVOLUTION OF HIPPOCAMPUS CellBio Final Lab Report 2020

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Background The scenario of this experiment is that my friends and I saw a herd of strange creatures at the seashore which had unusual fungus growing on them. One of them got tangled in a fishing line. We then got it free and decided to run a proteomic analysis of this new creature along with the other four samples which were Flying fish, Horse, Dolphin, and Salmon to determine where this unknown creature belongs to. Among five samples, Flying fish and Salmon are not mammals, whereas, Dolphin and Horse are mammals. Flying fish can be found in temperate and tropical water. They have a wing-like structure helping them to glide up. Flying fish breathes by gills and lays eggs. Among the samples, Salmon shares some characteristics with Flying fish such as gills for breathing and laying eggs. Besides Flying fish, Dolphin can be seen in both cold and temperate, tropical water. Dolphin is known as one of the most well studied and known marine mammals. Like other mammals, Dolphin gives birth to live offspring and breathes through lungs. In 5 samples, Horse is a mammal having the most characteristics the same as the Dolphin such as breathing through lungs or giving birth to live offspring. The only difference is that Horse only lives on land. And, the unknown creature, Hippocampus, is also known as a seahorse having an upper body of a horse and a lower body of a fish. They can be found in both freshwater and seawater. By having these characteristics of all five tested samples, proteomic analysis was performing to determine the evolution of Hippocampus. The study of protein is called proteomics. Proteomics includes the analysis of protein structure and function and how they interact with each other. Methods of studying protein are always involved in the purification and the isolation of the proteins. Purified proteins can be used for preparative use, which produces large quantities of the same protein for help, and analytical use, which extracts a small amount of protein. The method to obtain purified proteins is protein extraction. After having the purified proteins of the samples, the Bradford assay method was used to measure the protein concentration based on the absorption of light of the solute. The method uses a dye, Coomassie Blue G-250, whose chemical properties can bind and interact with the side chains, or R-group. As the dye binds and interacts, a blue color will be observed. A spectrophotometer is used to quantify the absorbance or the protein concentration of the samples at the wavelength of595nm

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THE EVOLUTION OF HIPPOCAMPUS kraken lab report. Proteomics Lab Report

THE EVOLUTION OF HIPPOCAMPUS CellBio Final Lab Report 2020 THE EVOLUTION OF HIPPOCAMPUS CellBio Final Lab Report 2020 kraken lab report. 2020 University of North Texas - BIOL 3520

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