BioChemistry > EXAM > BIOCHEM C785- 2 OA readiness check-with verified answers -2022 (All)

BIOCHEM C785- 2 OA readiness check-with verified answers -2022

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What is the complementary sequence to 3’ ATG CGA ATA 5’? (0/1 Points) 3’ TAT TCG CAT 5’ 3’ TAC GCT TAT 5’ The correct answer is 3' TAT TCG CAT 5'. Remember complementary means... “Matching or Pairing” You have to remember to pay attention to your numbers as well as your letters (A-T, G-C, 5'-3'). The correct answer is 3’ ATG CGA ATA 5’ (original sequence) 5’ TAC GCT TAT 3’ (complementary sequence) But we asked for it in the 3’ direction, so mirror answer to give correct answer: 3’ TAT TCG CAT 5’ If you chose 3' TAC GCT TAT 5', this is incorrect. Although the nucleotides are in the correct order, the 5' and 3' are in the wrong place. DNA strands that are complementary run in opposite directions. This means that if one strand starts with a 3', the the complementary strand must start with a 5'. If none of the answer choices give the correct answer in 5' ---> 3', then you need to write its mirror, which will run from 3' ---> 5'. 3’ GAT AGC ATA 5’ 5’ ATA AGC GTA 3’ 2 Which amino acid sequence would result from this Coding DNA strand? 3’ ATG CAG ATA 5’? (1/1 Points) Tyr Val Tyr Ile Gln Ile Ile Asp Val The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are non- complementary and parallel. So we copy the coding strand , change T ---> U, and then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’ mRNAMirror by changing orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val (chart is in direction of 5' ---> 3') Leu His Lys Tyr Tyr Arg Ile Met Ala Ser Val Ile The correct answer is Ser Val Ile because 5’ TAT TAC CGA 3’ template is complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val Ile Ser His Gln dNTPs, Primer, RNA Polymerase, template RNA CONTINUED.......... [Show More]

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