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BIOD 210 Exam module 5/ Genetics/Questions&Answers

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Consider the following DNA sequence: 3’-AAC TAC TGT GTA -5’ a. Write out the complementary RNA sequence, including the 5’ and 3’. b. Translation starts on which end of the RNA (5’ or 3’)? c. Identify the start codon in the complementary sequence you wrote in part a. of this question. Your Answer: a. 5’-UUG AUG ACA CAU -3’ b. 5’ c. AUG Question 2 2 / 2 pts True or False: Transcription of DNA into mRNA does not require an RNA primer. Corrrreecctt!! True False Question 3 3 / 6 pts Your Answer: Why must the DNA within eukaryotes be complexed with histone proteins, but not in bacteria? What happens to these complexes during DNA replication? Because in eukaryotes have ends on their DNA and bacteria is circular at the ends they have to be able to fuse so bacteria is unable to because of the circular shape the DNA unwinds and the nucleosome reforms and the histones proteins is very tightly coupled so new DNA strand can be wrapped up with new histones There is a huge amount of DNA within a eukaryotic cell, and it needs to be stored within a tiny space within the nucleus. Therefore, it is complexed with proteins into chromatin that help to condense it into an extremely compact form. When it is time for DNA replication to occur, the complexes relax due to the proteins dissociating from it. Then as soon as replication is complete at a certain point, the DNA winds around the histone proteins and recondenses. Question 4 10 / 10 pts Matching each term with the correct definition: Ribosomes Serves as a site for protein translation on the ER surface Corrrreecctt!! Shelterin complex Protects chromosome ends from degradation Corrrreecctt!! Corrrreecctt!! Codon Three nucleotides that form the code for an amino acid Reverse transcription Corrrreecctt!! Synthesis of DNA from a strand of RNA Exon mRNA sequences that are coding sequences for protein Corrrreecctt!! Question 5 6 / 6 pts Your Answer: Define the wobble hypothesis and explain why it is important for the degeneracy in coding. it is third base pair of triplet is less critical than the first two bases. its important for degeneracy coding because only the first two base pairs of a codon are ccritical forbinding to tRNA , the third can wobble so tRNA can recognize more than one triplet to encoe for a specific amino acid

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