ASCP BOC EXAM Study Guide

ASCP BOC EXAM Study Guide When using an electronic cell counter, which of the following results can occur in the presence of a cold agglutination? a) increased MCV and decreased RBC b) increased MCV and normal RBC c) ...

ASCP BOC EXAM Study Guide When using an electronic cell counter, which of the following results can occur in the presence of a cold agglutination? a) increased MCV and decreased RBC b) increased MCV and normal RBC c) decreased MCV and increased MCHC d) decreased MCV and RBC - ✔✔a) increased MCV and decreased RBC Cerebrospinal fluid for glucose assay should be: a) refrigerated b) analyzed immediately c) heated to 56 degree C d) stored at room temp after centrifugation - ✔✔b) analyzed immediately The major crossmatch will detect: a) group A patient mistyped as group O b) unexpected red cell antibody in the donor unit c) Rh-neg donor unit mislabeled as RhPos d) recipient antibody directly against antigens on the donor red cells - ✔✔d) recipient antibody directly against antigens on the donor red cells The anion gap is useful for quality control of laboratory reselts for a) amino acids and proteins b) blood gas anaylses c) sodium, potassium, chloride and total C02 d) calcium, phosphorus and magnesium - ✔✔c) sodium, potassium, chloride and total C02 A common cause of respiratory alkalosis is a) vomitting b) starvation c) asthma d) hyperventilation - ✔✔d) hyperventilation Blood is diluted 1:200, and a platelet count is performed. 180 platelets were counted in the center square millimeter on one side of the hemacytometer and 186 on the other side/ The total platelet count is: a) 146 x 10³/μL (146 x 10⁹/L) b)183 x 10³/μL (183 x 10⁹/L) c)366 x 10³/μL (3666 x 10⁹/L) d)732 x 10³/μL (732 x 10⁹/L) - ✔✔c)366 x 10³/μL (3666 x 10⁹/L) When staining acid-fast bacilli with Truant auramine-rhodamine stain, potassium permanganate is used a a) decolorizing agent b) quenching agent c) mordant d) dye - ✔✔b) quenching agent A first morning sputum is received for culture of acid-fast bacilli. It is digested and concentrated by the N-acetyl-L-cysteine alkali method. Two Sabouraud dextrose slants are incubated in the dark at 35ºC with 5%-10% CO2. The smear reveal acid-fast bacilli, but the slants show no growth after 8 weeks. The explanation is: a) improper media used b) incorrect concentration procedure used c) improper specimen submitted d) exposure to CO2 prevents growth - ✔✔a) improper media used MacConkey media for screening suspected cases of hemorrhagic E. coli O157:H7 must contain: a) indole b) citrate c) sorbitol d) lactose - ✔✔c) sorbitol A patient with a B-cell deficiency will most likely exhibit: a) decreased phagocytosis b) increased bacterial infections c) decreased complement levels d) increased complement levels - ✔✔b) increased bacterial infections Communication is enhanced by: a) a planned strategy that includes listening skills and ensuring an understanding with questions b) relying on e-mail, memos and voice mail to communicate new information c) formal, hierachical patterns instead of informal networking patterns d) assumptions if there are questions about the intent of the message - ✔✔a) a planned strategy that includes listening skills and ensuring an understanding with questions IgM antibodies are frequently hemolytic because of: a)the dimeric structure b) the molecule's 5 antigen binding sites c) their sedimentation coefficient of 7-15 S d) their coefficient ability to fix complement - ✔✔b) the molecule's 5 antigen binding sites A community hospital microbiology laboratory is processing significant numbers of stool cultures because of an outbreak of diarrhea following heavy rains and flooding in the county. A media that should be incorporated in the plating protocol is: a) colistin nalidixic acid for listeria b) MacConckey agar with sorbitol for Campylobacter c) mannitol salt agar for Enterococcus speices d) thiosulfate citrate bile salts sucrose for Vibrio species - ✔✔d) thiosulfate citrate bile salts sucrose for Vibrio species 80 grams of NaOH (MW=40) are how many moles? a) 1 b) 2 c) 3 d) 4 - ✔✔b) 2 An electrode has a silver/silver chloride anode and a platinum wire cathode. It is suspended in KCl solution and separated fro the blood to be analyzed by a selectively permeable membrane. Such an electrode is used to measure which of the following? a) pH b) PCO2 c) PO2 d) HCO2 - ✔✔c) PO2 The organism most commonly associated with neonatal purulent mentingitis is: a) Neisseria meningitidis b) Streptococcus pneumoniae c) group B streptococci d) Haemophilus influenzae - ✔✔c) group B streptococci The volume of urine excreted in a 24-hour period by an adult patient was 300mL. This condition would be termed: a) anuria b) oliguria c)polyuria d) dysuria - ✔✔b) oliguria Amniotic fluid is tested for the concentration of lamellar bodies. This test determines: a) fetal lung maturity (FLM) b) hemolytic disease of the newborn (HDN) c) alphafetoprotein (AFP) d) trisomy 21 - ✔✔a) fetal lung maturity The purpose of testing with anti-A,B is to detect: a) anti-A¹ b) anti-A² c) subgroups of A d) subgroups of B - ✔✔c) subgroups of A The most important buffer pair in plasma is the a) phosphate/biphosphate pair b) hemoglobin/imidazole pair c) bicarbonate/carbonic acid pair d) sulfate/bisulfate pair - ✔✔c) bicarbonate/carbonic acid pair Evidence of active red cell regeneration may be indicated on a blood smear by: a) basophilic stippling, nucleated RBC and polychromasia b) hypochromia, macrocytes and nucleated RBC c) hypochromia, basophilic stippling and nucleated RBC d) HowellJolly bodies, Cabot rings and basophilic stippling - ✔✔a) basophilic stippling, nucleated RBC and polychromasia Which of the following is most closely associated with chronic myelogenous leukemia? a) ringed sideroblasts b) disseminated intravascular coagulation c) micromegakaryocytes d) Philadelphia chromosome - ✔✔d) Philadelphia chromosome All stages of neutrophils are most likely to be seen in the peripheral blood of a patient with: - ✔✔(chronic granulocytic leukemia) CML to be analyzed by gas liquid chromatography a compound must - ✔✔be volatile or made volatile Rheumatoid factors are immunoglobulins with specificity for allotypic determinants located on the: - ✔✔Fc fragment of IgG. Substances that are ANTIGENIC ONLY when coupled to a protein carrier. - ✔✔haptens A technologist splashed a corrosive chemical in his/her eyes. To prevent permanent injury, the first action should be to: - ✔✔use the eyewash station to flush eyes with water for 15 minutes Which of the following immunoglobulins is the most efficient at agglutination? - ✔✔IgM Use of "standard" (universal) precautions minimizes exposure to: - ✔✔bloodborne pathogens Which of the following is true about direct ion selective electrodes for electrolytes? - ✔✔whole blood specimens are acceptable Leukocyte reduced red blood cells are ordered for a newly diagnosed bone marrow candidate . Which way is the best way to prepare this product. - ✔✔Transfuse through a log3 leukocyte removing filter the steam autoclave method of sterilization - ✔✔uses 15 lbs of pressure for 15 minutes Factors that contribute to a PCO2 electrode requiring 60-120 seconds to reach equilibrium include the: - ✔✔diffusion characteristics of the membrane Of the following, the disease most closely associated with pale blue inclusions in granulocytes and giant platelets is: - ✔✔May Hegglin Which of the follwing factors would make an organism appear to be more resistant on a disk diffusion sustibility test? - ✔✔too many organisms in the innoculum In spectrophotometry, the device that allows for a narrow band of wavelengths is the: - ✔✔Monochromator. Leukocyte-poor red blood cells would most likely be indicated for patients with a history of - ✔✔febrile transfusion reactions. In synovial fluid, the most characteristic microscopic finding in pseudogout is - ✔✔calcium pyrophosphate dihydrate crystals An index of precision is statistically known as the: - ✔✔coefficient of variation AHG coombs control cells : - ✔✔are coated with IgG antobody Which laboratory technique i