Portage Microbiology Module 2
Questions and Answers Rated A+
cellular metabolism ✔✔a controlled set of biochemical reactions that occur in living organisms in
order to maintain life
enzyme ✔✔a protein, or group of pr
...
Portage Microbiology Module 2
Questions and Answers Rated A+
cellular metabolism ✔✔a controlled set of biochemical reactions that occur in living organisms in
order to maintain life
enzyme ✔✔a protein, or group of proteins, that catalyze (speed up) chemical reactions/is not
consumed during the reaction and can be used repeatedly by the cell
cofactor ✔✔a small chemical component, usually metal ions, that assist enzymes during the
catalysis reactions/serve as regulators of chemical reactions: in their absence, enzymes are inactive
while in its presence enzymes are active
catabolism ✔✔the process of breaking down larger molecules into useful energy sources
anabolism ✔✔the building up or biosynthesis of macromolecules from smaller molecular units
into larger complexes
anabolism ✔✔used during growth and repair phases of the cell
ATP ✔✔most widely used form of energy in the cells
donate/accept ✔✔ATP has energy to _________ while ADP can ___________ energy (phosphate
group) to become ATP
phototrophs ✔✔acquire energy from photons of light to generate ATP from ADP
chemotrophs ✔✔acquire energy from preformed (already existing) chemicals found in the
environment
organotrophs ✔✔removing electrons from organic molecules such as glucose
lithotrophs ✔✔remove electrons from inorganic molecules such as elemental sulfur
carbon ✔✔in order to carry out metabolic processes, microbes must also have a source of...
heterotroph ✔✔a microorganism that derives its carbon from organic molecules such as sugars
autotroph ✔✔organisms that derive their carbon from inorganic molecules, most often from
carbon dioxide (CO2)
phosphorylation ✔✔the addition of a phosphate group
photophosphorylation ✔✔light energy is used to power the formation of ATP from ADP
substrate-level phosphorylation ✔✔the phosphoryl (PO3) group of a chemical compound is
transferred and donated (added) directly to ADP
phosphorylated reactive intermediate ✔✔in substrate-level phosphorylation, the chemical
compound losing the phosphate group is referred to as the...
oxidative phosphorylation ✔✔used by chemotrophs, the energy released by the chemical oxidation
of nutrients is used to reform ATP
glycolysis ✔✔the breakdown of a single molecule of glucose
reactants ✔✔molecules present and involved at the beginning of a chemical reaction
2NAD/2ATP ✔✔reactants with glucose in glycolysis
phosphorylation of glucose ✔✔prevents glucose from diffusing out of the cell as well serving as
the signal molecule to the cell that glycolysis is about to begin
products of glycolysis ✔✔2 pyruvate molecules, 2 NADH molecules and 4 ATP
NAD/NADH ✔✔plays a vital role in generating and maintaining energy for the cell
fermentation/respiration ✔✔two main strategies utilized by cells to replenish the supply of NAD+
fermentation ✔✔an anaerobic process in which NADH is converted back to NAD+ while pyruvate
is converted to a waste byproduct, commonly lactic acid or ethanol, to be eliminated from the cell
respiration ✔✔a more efficient aerobic process used by microorganisms to produce energy
tricarboxylic acid (TCA) cycle ✔✔central pathway of respiration/requires an additional coenzyme
similar to NAD called flavin adenine dinucleotide (FAD)
products of TCA ✔✔2 ATP in total (one for each pyruvate processed) and an abundance of
reduced electron carriers: NADH and FADH2
primary function of the TCA cycle ✔✔production of these reduced electron carriers (NADH and
FADH2)/the transfer of these electrons will fuel the generation of ATP via the electron transport
system
electron transport chain ✔✔a continuation of cellular respiration and can proceed either
aerobically or anaerobically
anaerobic respiration ✔✔less efficient and yields fewer ATP molecules than aerobic respiration
mitochondria ✔✔as electrons are transferred from NADH/FADH2 to terminal electron acceptors
(O2; aerobic respiration), energy is released and captured by electron acceptor proteins located in
the inner membrane of....
protons ✔✔electrons are then passed down a chain of electron acceptors (thus the name) causing
___________ (H+; positive charge) to be pumped out of the membrane, causing a strong
differential across the mitochondrial membrane,
proton motive force ✔✔drives H+ back through the ATP synthase complex, also located in the
membrane, resulting in the production of up to 34 molecules of ATP
polysaccharides ✔✔typically molecules that are too large to be transported directly across the
plasma membrane. Instead microbes must secrete enzymes capable of degrading the polymer into
small, more manageable subunits
direct contact ✔✔________________ w/ a polysaccharide is often required by the microorganism
to ensure maximal absorption
proteins ✔✔must also be broken into smaller subunits or individual amino acids by enzymes
protease ✔✔an enzyme that catabolizes protein
amino acid ✔✔a rich source of nutrients as they are a source not only of carbon and energy but
also nitrogen and sulfur
lipids ✔✔rich in energy, often having many reduced carbon molecules
lipase ✔✔separate the fatty acid chains from the glycerol backbone
lipid backbone ✔✔processed for carbon and energy
fatty acid chain ✔✔degraded via the β-oxidation pathway
β-oxidation pathway ✔✔a highly efficient process that overlaps with both the TCA and electron
transport chain cycles/for each lipid-derived carbon atom 48 molecules of ATP are yielded,
compared to the 38 molecules of ATP produced from glucose
photosynthesis ✔✔the process of capturing sunlight and converting it into the usable energy
sources ATP and NADPH
chloroplasts ✔✔site of photosynthesis, double membrane-enclosed organelles specific to algae
and plants
chlorophyll ✔✔photosynthetic pigment
photophosphorylation ✔✔also known as the 'light reactions', is the process of converting light
energy into chemical energy to be used by the cell in the forms of ATP and NADPH
membrane ✔✔light reactions always occur in the __________________, as one of the main
functions is to generate a proton concentration gradient to generate ATP, a function very similar
to the electron transport systems proton motor force
Calvin cycle ✔✔also referred to as the 'dark reactions', involves the ability of a microorganism to
use the ATP and NADPH generated in the light reactions to convert carbon dioxide (CO2) and
water (H2O) into organic carbon compounds and useful carbohydrates
carbon fixation ✔✔to convert carbon dioxide (CO2) and water (H2O) into organic carbon
compounds and useful carbohydrates
primary function of Calvin cycle ✔✔to produce the three-carbon compound glyceraldehyde 3-
phosphate (G3P), which, through a series of steps, eventually leads to the generation of glucose
end byproducts ✔✔__________________ of the Calvin cycle supplies the molecules required
during the light reactions, just as the end products of the light reactions enables the dark reactions
to occur
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