Module 2- Microbiology Portage
Learning Questions and Answers 100%
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cellular metabolism ✔✔a controlled set of biochemical reactions that occur in living organisms to
maintain life
Enzymes ✔✔a protein, or group o
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Module 2- Microbiology Portage
Learning Questions and Answers 100%
Pass
cellular metabolism ✔✔a controlled set of biochemical reactions that occur in living organisms to
maintain life
Enzymes ✔✔a protein, or group of proteins, that speed up (catalyze) chemical reactions
Cofactors ✔✔a small chemical component, usually metal ions, that assist and regulate enzymes
during the catalysis reactions.
enzymes break down proteins into what: ✔✔amino acids
enzymes break down lipids into what: ✔✔glycerrol/fatty acids
enzymes break down polysaccharides into what: ✔✔monosaccharides
Anabolism ✔✔the building up of macromolecules from smaller molecular units into larger
complexes.
Catabolism ✔✔Metabolic pathways that break down molecules, releasing energy.
Phototrophs ✔✔organisms that get energy from photons of light to generate ATP from ADP
Chemotrophs ✔✔organisms that obtain energy from chemicals found in the environment
Organotrophs ✔✔chemotrophs that remove electrons from organic compounds (such as glucose)
Lithotrophs ✔✔chemotrophs that remove electrons from inorganic molecules (such as elemental
sulfur)
Heterotrophs ✔✔a microorganism that cannot make its own food and must get carbon from
organic molecules (ie. sugars)
autotrophs ✔✔Organisms that make their own food and derive their carbon from inorganic
molecules (most often CO2)
Phosphorylation ✔✔The transfer of a phosphate group, usually from ATP, to a molecule. Nearly
all cellular work depends on ATP energizing other molecules by phosphorylation.
3 Types of phosphorylation reaction that generates ATP: ✔✔1. Photophosphorylation - the use of
light energy / 2. Substrate-level phosphorylation - the phosphoryl (PO3) group of a chemical
compound is transferred and donated directly to ADP / 3. Oxidative phosphorylation - the energy
released by the chemical oxidation of nutrients is used to reform ATP
glucose metabolism ✔✔The process by which cells commonly obtain energy by breaking the
chemical bonds of glucose molecules and use this energy to form ATP. Yields up to 38 ATP and
involves three distinct transitions.
steps of glucose catabolism ✔✔1. Glycolysis: the breakdown of glucose to pyruvate to yield
energy. Yields 2 ATP
2. Either fermentation or respiration yields 2 additional molecules of ATP
3. Electron Transport Chain produces 34 ATP via an oxidative phosphorylation event at the plasma
membrane
Reactants ✔✔Any molecules present and involved at the beginning of a chemical reaction.
Located to the left of the reaction arrow when written out.
Glycolysis ✔✔The first step in releasing the energy of glucose, a single glucose molecule is broken
down into two pyruvate molecules. As a result of this process, NADH and ATP are also produced.
Can occur in the presence or absence of oxygen.
fermentation (anaerobic respiration) ✔✔the breakdown of sugars without the use of oxygen,
regenerate NAD+ so glycolysis can continue
cellular respiration ✔✔Process that releases energy by breaking down glucose and other food
molecules in the presence of oxygen
Krebs Cycle (Citric Acid Cycle) ✔✔second stage of cellular respiration in eukaryotes, takes place
in matrix of mitochondria,
- Pyruvic acid combines with coenzyme A to form Acetyl-CoA which enters Krebs cycle
- in matrix of mitochondria
- each turn of Krebs cycle produces 1 molecule of ATP, 1 molecule of FADH2, and 3 molecules
of NADH (multiply to 2 since there are two Pyruvates
electron transport chain ✔✔third stage of cellular respiration
NADH is oxidized to form NAD+ and FADH2 is oxidized to form FAD. As the electrons from
these carriers move down the ETC, energy is released. This energy pumps H+ ions across the
membrane, creating a proton gradient which is used for chemiosmosis and generates ATP.
proton-motive force ✔✔The potential energy stored in the form of an electrochemical gradient,
generated by the pumping of hydrogen ions across biological membranes during chemiosmosis.
beta oxidation ✔✔A metabolic sequence that breaks fatty acids down in the cytosol in prokaryotes
and in the mitochondria in eukaryotes to generate AcetylCoA, which enters the Krebs cycle.
Photosynthesis ✔✔Conversion of light energy from the sun into chemical energy.
Chloroplast ✔✔An organelle found in plant and algae cells where photosynthesis occurs
Photophosphorylation ✔✔The light reaction of photosynthesis, and is the process of converting
light energy into chemical energy to be used by the cell in the forms of ATP and NADPH., occurs
in the membrane
Calvin Cycle ✔✔the dark reaction of photosynthesis (light independant) and involves the ability
of a microorganism to use the ATP and NADPH generated in the light reaction to convert CO2
and H2O into organic carbon compounds and useful carbohydrates, requires 6 turns to generate
one molecule of glucose
Substrate ✔✔The reactant on which an enzyme acts on.
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