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Portage Microbiology Lab Exam 1 Latest 2022 Rated A+ What three elements are used in an autoclave to sterilize equipment? ✔✔1. Heat 2. Pressure 3. Steam What type of incubator is pictured below? ✔✔The picture below i ...

Portage Microbiology Lab Exam 1 Latest 2022 Rated A+ What three elements are used in an autoclave to sterilize equipment? ✔✔1. Heat 2. Pressure 3. Steam What type of incubator is pictured below? ✔✔The picture below is a Fixed Incubator. What is the minimum temperature an autoclave must be set at to achieve sterile condition? ✔✔The minimum temperature for an autoclave to reach sterile conditions is 125°C. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you choose to best sterilize your equipment? Hot steam or hot air? Explain why you chose your answer. ✔✔The best way to sterilize equipment if an autoclave is not available, it should be hot steam because it will sterilize the equipment in a matter of minutes while hot air could take hours to reach the same effect. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial. ✔✔4°C. This temperature slows bacterial growth but does not stop it, thereby preserving the sample. What are the FOUR types of gloves presented in the lab video? ✔✔Latex gloves, Nitrile gloves, Thermal cold gloves, Thermal heat gloves. At what temperature is the fixed incubator set to, as presented in the lab video? ✔✔The fixed incubator should have a temperature of 37°C. What THREE rules were discussed in regards to lab safety that would protect you and others from contamination? ✔✔1. Never bring any food or drinks inside lab settings 2. Always use PPE in lab settings 3. Never leave lab settings while using PPE What are the main sections that should be found in a lab notebook? Name at least 4. ✔✔1. Objectives 2. Procedure 3. Notes 4. Results 5. Deviations 6. Lab Title You are a lab instructor, and Paul Smith has turned in his lab notebook for you to grade the lab experiment #2 on microscopy. Based upon what was covered in the lab video, how should Paul have titled his lab experiment? ✔✔PS02 Microscopy (1) According to the lab lecture, under what section of his notebook would you look to find the experimental steps? (2) As changes to the experimental steps were made, what are these differences called, and how should it appear in the lab notebook? ✔✔1. The experimental protocol will be found in the "Procedure" section of the lab notebook. 2. Any changes to protocol are called deviations and should appear in red ink within the "Procedure" section.

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