Micro Biology > EXAM > Bacterial Identification Lab / EXAM / 100% VERIFIED ANSWERS (All)

Bacterial Identification Lab / EXAM / 100% VERIFIED ANSWERS

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To familiarize students with the science and technology used to identify types of DNA based on their DNA sequence. - ANS -What is the overall purpose of this virtual lab? Prepare a sample and isola... te the whole DNA, make copies of it, sequence the DNA, analyze and identify - ANS -What are the four basic steps involved in this bacterial identification lab? The piece of DNA used for identifying bacteria; The identification relies on matching the sequence from your sample against a database of all known 16S rDNA sequences - ANS -What is "16S rDNA," and how is it used to identify species of bacteria? to identify a bacterial sample received from a clinician. - ANS -As the pathology lab technician, what is your task in this virtual lab? Extracting DNA involves which initial step? - ANS- dissolving the cell wall with a digestive buffer (in the white-capped bottle) available as a commercial kit What is the wire ring used for? - ANS -The buffer contains proteolytic enzymes that eat the cell wall. - ANS -Why are the proteolytic enzymes necessary? Why do you then need to inactivate the proteolytic enzymes and how do you do it? - ANS - Since we will be using other enzymes in the next step, we need to get rid of the proteolytic enzymes Why do you spin the sample in the centrifuge? - ANS -DNA is lighter than the cellular debris floating around from the step that destroyed the cellular wall. The centrifuge allows us to safely remove the DNA from the microcentrifuge tube. What is the pellet? - ANS - After removing the enzymes a solid deposit of cellular debris What is the supernatant? - ANS - the liquid Where is the DNA? - ANS - the DNA is contained in the supernatant What does "PCR" stand for and what is the purpose of PCR? - ANS - Polymerase Chain Reactant; makes copies of DNA - ANS -Summarize the process of PCR in a diagram. Include all the steps, labeled and in the right order. [Show More]

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