Biology > QUESTIONS & ANSWERS > University of Minnesota-Twin Cities BIOL 4003 Exam 2. (All)

University of Minnesota-Twin Cities BIOL 4003 Exam 2.

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Practice Questions for the Exam: T/F: 1) T F In eukaryotes both transcription and translation occur in the same cellular compartment. 2) T F DNA replication is primed by RNA primers, which are sub... sequently removed and replaced with DNA. 3) T F Transcription is the process by which mRNA is read by ribosomes to produce protein. 4). T F GC-rich DNA melts at a lower temperature than AT-rich DNA. 5) T F The binding of TBP (TATA binding protein) to a sequence 30 sequences upstream of the start site of transcription plays a key role in the initiation of transcription in eukaryotes. 6) T F Translation in prokaryotes begins before transcription is terminated. 7)T F The most conserved sequences that characterize introns (expressed as RNA sequence are GU at the 5’ end of the intron and AG at the 3’ end of the intron. 8) T F In DNA, pyrimidines base pair with purines 9) T F The highly repeated sequence at telomeres is template by the sequence of an RNA that is an integral part of telomerase. 10) T F In HFr x F- matings most of the F- recipient cells become F+ 11) In the Meselson and Stahl experiment that demonstrated that DNA replication is semi-conservative, heavy nitrogen (N15) was used to change the density of DNA during ultracentrifugation. Cells were labeled for many generations in N15 and then washed into N14 (normal or “light” nitrogen) medium for different numbers of cell cycles. They observed three types of DNA in their experiments: heavy (N15/N15); hybrid (N15/N14) and light (N14/N14). When cells were washed from N15 to N14 and grown for one generation, all of the DNA banded at the hybrid density. After a second generation in N14, what density band or bands were observed: a) On band, hybrid density; b) Two bands (one heavy, one light); c) Two bands (one hybrid, one light); d) Three bands (one of each density); e) None of the above [Show More]

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