MB (ASCP) 2022 Already Passed

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MB (ASCP) 2022 Already Passed Consider the table below where a child and a possible father (PF) share the listed paternity indices for each locus listed (LOC-A1, LOC-B2, LOC-C3, LOC-D4). Locus Test ... ed PF Child Paternity Index LOC-A1 3 2/3 2.18 LOC-B2 7/5 5 0.798 LOC-C3 15/17 9/17 5.21 LOC-D4 12 12 1.37 Based on the data presented in the table, what is the combined paternity index, CPI, from the loci tested: LOC-A1, LOC-B2, LOC-C3 and LOC-D4? 12.42 9.558 15.56 2.38 12.42 ( CPI is calculated by multiplying all Paternity index tgt) Best way to avoid PCR contamination Separate Pre-PCR (clean area) and post PCR (dirty area) A260=1.4, A280=1.1 . protein contamination or Rna contam or useable RNA Protein contamination . A260/280 = 1.3 <1.6 = protein contamination, 1.7-2.0 = good DNA, 1.9-2.0 = good RNA Why is Fluorometry a better technique that Spectroscopy Increased sensitivity and more importantly can accurately measure DNA concentration of a structurally intact double stranded DNA molecule Linkage Disequilibrium LD is the non random association of alleles at 2 or more loci in a general population. When alleles are in LD, haplotypes do not occur at the expected frequencies. Specificity Specificity = TN / (TN + FP) Limit of Detection (LOD) lowest concentration of analyte that can be detected mRNA Sequence provided - Convert it into DNA (Template/Anti-sense Strand) [Replace U with T] Correct order of HIV Sample Preparation Workflow Plasma Preparation RNA Extraction RNA Amplification/Modification Procedure If a flask of cells is provided for the intended purpose of DNA isolation, how would you proceed further Trypsinize and/or scrape the cells inside the provided flas [Show More]

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