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ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: ✔✔to label DNA probes by random primer labeling. What is a solution hybridization method for the detecti ...

ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: ✔✔to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? ✔✔gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucleic acid interaction. ✔✔Gel mobility shift assay Which probe is most often used to detect trisomy 21 by interphase FISH? ✔✔alpha satellite DNA probe _____ detect specific chromosomes and numbers of the specific chromosomes. ✔✔Alpha satellite DNA probe Which reagent generates a signal during the annealing stage of a quantitative real time PCR reaction? ✔✔molecular beacon Methylation of cytosine residues is involved in the alteration of gene expression in a number of cancers. Which sequencing technique is best suited to determine if methylation of cytosine residues has taken place in the promoter region of a given gene? ✔✔bisulfite sequencing _____ is a modified chain terminator method specifically designed to detect methylated nucleotides. ✔✔Bisulfite sequencing Which method is best suited to screen for mutations in a gene having multiple possible mutations? ✔✔single strand conformational polymorphism (SSCP) _____ is a screening test in which the exact nature of a mutation does not need to be known in order to detect the presence of a mutation. ✔✔Single strand conformational polymorphism (SSCP) What is the best method to rule out the possibility of genomic DNA giving a false-positive result in an RT-PCR reaction? ✔✔use of upstream and downstream primers that span an exon-intronexon region of the target *amplicaons generated from genomic DNA would be larger than the specific amplicon generated from a mature, spliced mRNA A common use for pulsed-field gel electrophoresis is: ✔✔epidemiological typing of bacterial strains. _____ after digestion of bacterial chromosomes with restriction enzymes is used to determine similarity between bacterial isolates. ✔✔PFGE A translocation in which chromosome pair creates a BCR/ABL1 fusion gene product associated with CML?

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