MB (ASCP) 2022 - Translocations, Diseases, Pharma, Polymerases, & more

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MB (ASCP) 2022 - Translocations, Diseases, Pharma, Polymerases, & more Chronic Myeloid Leukemia (CML) ✔✔t(9;22) BCR-ABL translocation Ewing sarcoma ✔✔t(11;22) EWS-FLI1 Acute Lymphocytic ... Leukemia (ALL) ✔✔t(9;22) BCR-ABL p190 protein Follicular Lymphoma ✔✔t(14;18) BCL2 translocation Mantle Cell Lymphoma ✔✔t(11;14) bcl-1 translocation CCND1-IgH Burkitt Lymphoma ✔✔t(8;14) MYC gene translocation Rhabdomyosarcoma (alveolar); Cancer/solid tumor ✔✔t(2;13) or t(1;13) PAX3-FKHR and PAX7-FKHR "ARMS" translocation AML (Acute Myeloid Leukemia) ✔✔t(8;21) inv(16) t(15;17) RUNX1, RUNX1T1 APL (Acute Promyelocytic Leukemia) also AML-3 ✔✔t(15;17) note: A *P* L = *P* ML-RARA (remember the P) Marginal Zone Lymphoma ✔✔t(11;18) Prostatic adenocarcinoma ✔✔t(7;21) or t(17;21) Sezary Syndrome (Cutaneous T-Cell Lymphomia) ✔✔TCR gene rearrangements Synovial sarcoma ✔✔t(X;18) SYT-SSX Breast Cancer ✔✔HER2/ERBB2 - BRCA1 mutation: 185delAG and 5832insC - BRCA2 mutation: 6174delT Lung Cancer (NSCLC) ✔✔EGFR mutation = L858R also Thr790Met Colorectal Carcinoma (CRC) ✔✔BRAF V600E mutation also PIK3CA mutation (E542 and E545 in exon 9) Melanoma (skin cancer) ✔✔BRAF V600E mutation (c.1799T>A) Target amplification methods ✔✔PCR + qPCR RT-PCR TMA (remember: both RT-PCR and TMA use reverse transcriptase) Probe Amplification methods ✔✔SDA LCR QB Replicase Signal Amplification methods ✔✔Hybrid Capture bDNA (branched DNA amplification) Cleavage-based (Invader assay) What is a metagenome? ✔✔sum total of all DNA sequenced from an environmental sample Which Enteroccus Faecium has the most resistance with MIC > 128 ug/ml? ✔✔vanA staphylococcus aureus (MRSA) What antimicrobial agent + genes conferring resistance? ✔✔Oxacillin mecA Streptococcus pneumoniae ✔✔IytA gene steptococcus pneumoniae What antimicrobial agent + genes conferring resistance? ✔✔Penicillin pbp1a and pbp1b Enterococcus What antimicrobial agent + genes conferring resista [Show More]

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