MLS ASCP Microbiology Questions and Answers Rated A

MLS ASCP Microbiology Questions and Answers Rated A 1 : 10 ratio ✔✔The proper blood-to-broth ratio for blood cultures to reduce the antibacterial effects of serum Beta-lactamase ✔✔Produced by a penicillin-resistant N ...

MLS ASCP Microbiology Questions and Answers Rated A 1 : 10 ratio ✔✔The proper blood-to-broth ratio for blood cultures to reduce the antibacterial effects of serum Beta-lactamase ✔✔Produced by a penicillin-resistant N. gonorrhoeae Columbia CNA with 5% sheep blood ✔✔Selective medium used for the isolation of grampositive microorgnisms Indole test ✔✔used for determining an organism's ability to produce indole from deamination of tryptophan JEMBEC system ✔✔is a transport and inoculation medium used for direct plating of specimens for N gonorrhoeae. Chocolate based selective medium is inoculated with the specimen. This is placed in an impermeable plastic bag with a bicarbonate-sodium citrate pellet to generate a carbon dioxide rich environment. Martin Lewis agar ✔✔Chocolate agar base containing vancomycin, colistin, anisomycin, and trimethoprim for better inhibition of gram-positive organisms and candida. Differential medium ✔✔aids in the presumptive identification of organisms based on their apperance SPS ✔✔used as anticoagulant in blood cultures to inactivate neutrophils and components of serum complement Blood culture ✔✔Typhoid fever can be confirmed by this culture Regan-Lowe agar ✔✔best medium for culture for Bordetella pertussis Francisella tularensis ✔✔most rapid method for detection is PCR synovial infections ✔✔Anaerobic susceptibility testing is helpful Schlichter test ✔✔is used to monitor bactericidal activity during antimicrobic therapy in cases of endocaditis Moraxella osloensis ✔✔is a gram-negative coccobacillus that is often plump and occurs in pairs and demonstrates a morphology similar to Neisseria Extra-intestinal isolates of Salmonella ✔✔should be tested for resistance to nalidixic acid and if resistant report the ciprofloxacin as resistant Gentamicin ✔✔antimicrobial resistance occurs from enzymatic drug modification Enterococcus infection ✔✔need 2 antimicrobial agents, penicillin derivative and an aminoglycoside pulsed-field gel electrophoresis ✔✔commonly used method to determine the relatedness of 2 or more bacterial strains Micrococcus ✔✔is modified oxidase positive, bacitracin (0.04U) susceptible and resistant to lysotaphin Enterococcus ✔✔may be used as a positive quality control for the bile esculin test Campylobacter ✔✔are gram-negative, curved bacilli "gull wings" that require microaerophilic conditions for growth Porphyrin test ✔✔was devised to detect strains of Haemophilus capable of synthesis of hemin Capnocytophage ✔✔Colonies are larger and spreading after 2-4 days HACEK group ✔✔organisms that require 5%-10% CO2 for growth SPS in blood culture media ✔✔Peptostreptpcoccus anaerobius is inhibited Costridium difficile toxin ✔✔can be detected by EIA Suspected diagnosis of subacute bacterial endocarditis ✔✔presumptive id by catalase and spot indole Actimomyces ✔✔Are yellow-tan and have a molar tooth appearance Bacteroides fragilis ✔✔common anaerobic bacteria isolated from infectious processes of soft tissue and anaerobic bacteremia Bacteroides ✔✔grows on BBE agar and produces black colonies Veillonella and Prevotella ✔✔organisms may inhibit a brick red fluorescence Fusobacterium nucleatum ✔✔A thin, gram-negative bacillus with tapered ends Bacteroides fragilis ✔✔is negative for idole Costridium perfringens ✔✔could be used as the positive QC test for lecithinase on egg yolk agar Cryptococcus neoformans ✔✔only yeast that produces brown colonies in birdseed agar; also only yeast that is phenol oxidase positive Malassezia furfur ✔✔causes catherer-related sepsis, requires olive oil discs for growth, and is a small yeast with a wide bud Chromogenic agar ✔✔is extremely effective in detecting mixed yeast populations Coccidioides immitis ✔✔id by irregular staining, barrel-shaped arthrospores Dermatophytes ✔✔differentiated by their macroconidia and microconidia Pseudallescheria boydii ✔✔common cause of eumycotic mycetoma Review the digestion and decontamination procedure ✔✔if noted that there fewer isolates of Mycobacterium being isolated than previous year M tuberculosis ✔✔will give a positive niacin test; id by rough, nonpigmented colony, slow growth at 37 C, nitrate reduction-positive M bovis ✔✔includes a BCG strain used for vaccination against tuberculosis M gordonae ✔✔contamination by water organisms M scrofulaceum ✔✔when grown in the dark, yellow to orange pigmentation of the colonies and are scotochromogens M xenopi ✔✔A nonchromogen that grows at 42C and is highly resistant to antibiotics diagnosis of gonorrhea ✔✔shows typical gram-negative intracellular diplococci. Growth on selective agar shows gram-neg diplococci Gram stain, motibility(25C), and catalase ✔✔Performed to differentiate between Listeria species and Streptococcus species S. aureus ✔✔coagulase properties, staphylococci isolates showing a positive tube test Coccidiodes immitis ✔✔The mycelial form of which dimorphic mold produces thick-walled, rectangular arthtoconidia Cryptosporidium species ✔✔shows pink-stained oocysts with modified Ziehl-Neelsen acid fast stain