MLS ASCP Certification Questions and
Answers 100% Pass
Indole Positive and Negative example. ✔✔Positive: E. coli
Negative Kleb. pneumo
Methyl red Positive and Negative example. ✔✔Positive: E. coli
Negative: Kleb. pn
...
MLS ASCP Certification Questions and
Answers 100% Pass
Indole Positive and Negative example. ✔✔Positive: E. coli
Negative Kleb. pneumo
Methyl red Positive and Negative example. ✔✔Positive: E. coli
Negative: Kleb. pneumo
Citrate Positive and Negative examples. ✔✔Positive: Kleb. pneumo
Negative: E. coli
VP and Citrate reactions for E. coli and Kleb. pneumo: ✔✔E. coli: negative
Kleb. pneumo: positive
Eosinophils in urine sediment indicate: ✔✔acute interstitial nephritis
What is measured in procainamide? ✔✔N-acetylprocainamide (NAPA)
Which microorganism is linked to glomerulonephritis? ✔✔Post-streptococcal: S. pyogenes
Group A
What diseases are linked to basophilic stippling? ✔✔-beta thal major
-lead poisoning
Organisms that must be worked on under the hood: ✔✔-Brucella
-N. meningitidis
-Mycobacterium
-Fungi and Molds
Who discovered ABO groups? ✔✔Karl Landsteiner
Beer's Law states that: ✔✔absorbance is directly proportional to the concentration of substance
in solution
Positive and Negative Control for Oxidase. ✔✔P. aeruginosa - pos
E. coli - neg
Positive and Negative control for butyrate: ✔✔Pos: M. catarhalis
Neg: N. gonorrhoeae
How do you differentiate Group D Strep from enterococcus? ✔✔-Both are Bile Esculin pos
-6.5% NaCl: Gp D is neg
Enterococcus is pos
PYR: Enterococcus is pos
Group D is neg
Major sources of ALP: ✔✔Heat stability: Placenta>intestine>liver>bone
ALP is elevated in: ✔✔1. bile duct obstruction (gallstone)
2. Cirrhosis
3. Hepatic tumors
4. ALT/AST>ALP-->hepatitis
On admission and 4 hours later what is the best test to confirm an AMI? ✔✔Troponin I
PT measures which factors? ✔✔Extrinsic: VII
Common: I, II, V, X
APTT measures which factors? ✔✔Intrinsic: 8, 9, 11, 12, prekallikrein
Common: 1, II, V, X
What confirs immunity from hepatitis B? If you have the immune marker what marker will you
have if you have had the infection along with the immune antibody? ✔✔Anti-HBs
Anti-HBc*distinguishes past infection vs just vaccination
Procedure for Using a Winged Blood Collection Device to Collect a Specimen for Coagulation
Tests: ✔✔Discard a blue top then use 2nd blue to get rid of air
Football shaped egg with hyaline plugs at each end: ✔✔Trichuris trichiura
Up to 4 nuclei, chromatoidal bars, vacuoles: ✔✔E. histolytica cyst
*trophozoites ingest RBCs..if you see this you can call it histolytica!
Hypersegmented neutrophils are seen in: ✔✔Megaloblastic anemias: Vit B12 or Folate
deficiency
Triple phosphate crystals are found in _______ urine and look like ______. ✔✔alkaline; coffin
lids
Tumor marker for colon cancer: ✔✔CEA
To determine peak levels, collect specimens after: ✔✔-IV:15-30 minutes
IM: >2 hours
Oral: 1-2 hours
Bacitracin test can also be used to differentiate: ✔✔bacitracin-resistant Staphylococcus from
bacitracin-susceptible Micrococcus
Primary marker for distinguishing pyelonephritis (upper UTI) from cystitis (lower UTI)
✔✔WBC cast
Waxy casts are more easily visualized than hyaline casts because of their: ✔✔higher refractive
index
associated with prolonged vomiting ✔✔Metabolic Alkalosis (Cl- loss so HCO3- retention)
Associated with severe diarrhea: ✔✔Metabolic Acidosis-losing bicarb..also associated with
salicylate poisoning and diabetic ketoacidosis
Disease associated with echinocytes? Artifact? ✔✔Uremia; didn't dry blood fast enough or
completely
The parathyroid gland releases PTH to __________ Ca2+ and ____PO4- ✔✔increase ; eliminate
PT, PTT and TT are all high ✔✔Fibrinogen
Nontreponemal test for syphillis ✔✔CSF VDRL
Cushing's ✔✔
Donor Requirements: ✔✔-Every 8 weeks (x2 if dbl unit) or >2 days after plateletpheresis
-pulse 50-100
-Temp = 37.5C/99.5F
-Hgb/Hct: >/= 12.5, 38%
Auto >/= 11, 33%
P. mirabilis is indole ___ , P. vulgaris is indole ___ ✔✔neg; pos
Mothball ingestion causes: ✔✔Heinz bodies
Hemoglobinuria
Methemoglobinemia
Blood positive on urine dipstick but no RBCS seen under the scope causes: ✔✔-Hypotonic and
alkaline causes RBC lysis.
-Myoglobin
-Bleach
Blood negative on urine dipstick but RBCS seen under the scope causes: ✔✔False negative:
Vitamin C and not mixing
Partial D is the result of a ___________. ✔✔Mutation in RHD gene
A change in the expression of D antigen is called _____. ✔✔Partial D
False negative urobilinogen urine strip: ✔✔Light
Formalin
False positive urobilinogen urine strip: ✔✔Anything that reacts with Ehrlich's reagents" PBG
and sulfonamides
Conn's Syndrome effects: ✔✔Increased aldosterone= ++Na+ and BPand blood volume and
decreased K+
What can elevate the SG? ✔✔High protein, glucose, bilirubin
(LMWH) Low molecular weight heparin therapy is monitored by: ✔✔Anti-Xa assay
Possible causes of PT, APTT, and TT being prolonged: ✔✔1. Afibrinogenemia
2. dysfibrinogenemia
3. DIC
4. Heparin contamination
5. Thrombin inhibitors
Urinary antigen testing: ✔✔Legionella pneumo
Strep pneumo
Histoplasma capsulatum
Target cells are seen in: ✔✔Lt Sh.
Liver disease
thalassemia
splenectomy
Hb C disease
Sporothrix schenckii ✔✔"Rose Gardner's Disease"
Conidophores look like a daisy
Cigar shaped yeast
Hydatid cyst fluid ✔✔Echinococcus granulosus
neutralize anti-P1
Excessive antacid use causes: ✔✔-Hypermagnesia
-Hypophosphotemia
Mg in the antacid binds PO4 in blood and prevents it from being absorbed
-Aluminum toxicity?
Review ouchterlony plate/double immunodiffusion. ✔✔Page 225 Micro book
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