SBB study guide questions with certified solutions

SBB study guide questions with certified solutions What enzyme may be useful in distinguishing anti-Ge2 from anti-Ge3? ✔✔Papain (Anti-Ge2 will not react) The beta 1-3 linkage between galactose and N-actylglucosamine ...

SBB study guide questions with certified solutions What enzyme may be useful in distinguishing anti-Ge2 from anti-Ge3? ✔✔Papain (Anti-Ge2 will not react) The beta 1-3 linkage between galactose and N-actylglucosamine is found in: ✔✔Secretions Which of the following represents the Type II chain? ✔✔Beta 1-4 linkage between galactose and N acetyl glucosamine Rank the order of decreasing H concentration on RBCs ✔✔0>A2>B>A2B>A1>A1B All of the following are genotypes for the A1 phenotype except: 1. A1/A1 2. A1/A2 3. A2/B 4. A1/O ✔✔A2/B The ABO genes are located on which chromosome? ✔✔9 Which glycosyltransferase is needed before A and B antigens are formed? ✔✔Lfucosyltransferase What is the typical reaction of the A3 subgroup? ✔✔mixed field reaction with Anti-A and AntiA,B What is the typical reaction of the Ael subgroup? ✔✔Positive reaction with anti-H What clinical condition may yield positive reactions with Anti-A, negative with Anti-B, negative with A1 and B cells. ✔✔Hypogammaglobulinemia What differentiates a Bombay anti-H from an anti-H cold agglutin? ✔✔Reactivity at body temperature The is antigen is associated with linear carbohydrate chains? ✔✔i antigen The following reactions are found on a 35 yr old male. Anti-A 4+, Anti-B 0, Anti-A,B-4+, A1 cells 3+, B cells 4+, O cells 3+. How could these be explained? ✔✔Anti-M in plasma Anti-IH would react best with which RBC? ✔✔O The acquired B phenomenon is only found in which ABO group? ✔✔A Which of the following is true about the A2 subgroup? 1. 1-8% of persons will product anti-A1 2. Anti-A1 does not react with A2 RBCs 3. Anti-A2 is formed 4. Two of the above ✔✔Two of the above (1-8% of persons will product anti-A1 and Anti-A1 does not react with A2 RBC ) Anti-Lu3 is associated with: ✔✔Recessive type Lu(a-b-) The dominant Lu(a-b-) type is associated with what gene? ✔✔In(Lu) What substance could be used to rule out anti-E in the presence of anti-Lub? ✔✔DTT Which pair of alleles is out of place? 1. Lu8/Lu14 2. Lu8/Lu10 3.Lu6/Lu9 4. Aua/Aub ✔✔Lu8/Lu10 Persons who are group B are more common among which population? ✔✔Black/Asian Red cells that lack glycophrin A also lack: ✔✔Ena antigens Anti-ENKT reacts with which RBCs? ✔✔ENaFS What is a lectin? ✔✔Seed extracts that agglutinate human cells with moderate specificity All of the following are representative of Lewis antibodies, except? 1. They can be neutralized by Lewis substance 2. They occur frequently in pregnant women 3. They are destroyed by enzymes 4. They are enhanced by enzymes ✔✔They are destroyed by enzymes Why can anti-H sometimes be fund in A2B persons? ✔✔The specific immunodominant sugar blocks the presence of H antigen Why is determenantion of Rh status crucial for obstetric patients? ✔✔All Rh negative mothers are possible candidates for Rhogam An adult who inherits Le,Se and H will type as: ✔✔Le(a-b+) What immunodominant sugar is responsible for B specificity? ✔✔D galactose Red cells that phenotype as Le(a-b-) will convert to Le(a+b-) when incubated with plasma from an adult containing? ✔✔Le(a+) substance What genotype does hh represent? ✔✔Bombay The Rh gene is located on chromosome? ✔✔1 Why are Lewis antibodies no implicated in hemolytic disease of the newborn? ✔✔Lewis antigens are poorly developed at birth What substances will be present in the saliva of a person with the LeLe HH SeSe AA genotype? ✔✔A, H, Lea, Leb How is Le(b) substance formed? ✔✔L fucose is added to the subterminal N acetylglucosamine of type 1H substance A person with the Oh phenotype can only receive RBC from: ✔✔Only Oh persons What amino acid is specific for the S antigen? ✔✔Methionine Which of the following is true regarding the U antigen? ✔✔Most U negative RBC are also S-s- Why is it imperative that homozygous cells be employed when ruling out Kidd antibodies? ✔✔Anti-Jka may appear compatible with heterozygous cells Anti-I is associated with what infectious agent? ✔✔Mycoplasma pneumonia What Kidd antibody wil react with all panel cess, but not the autocontrol? ✔✔Anti-Jk3 All of the following are characterists of anti-Fya and anti-Fyb, EXCEPT: 1. can cause HDFN 2. Reacts best in AHG 3. enhanced with enzymes 4. IgG ✔✔Enhanced with enzymes This antibody typically causes RBC to exhibit a refractile shiny appearance and mixed field reaction at AHG, and can be neutralized by most human urine? ✔✔Anti-Sda What solution can denature Bennet-Goodspeed antigens? ✔✔Cholorquine diphosphate Persons with the McLeod phenotype do not express this marker. ✔✔Kx DTT will destroy antigens from which blood group? ✔✔Kell Chronic granulomatous disease (CGD) is associated with what phenotype? ✔✔McLeod What P antigens are present in the p phenotype? ✔✔none What antigens are present in the P1 phenotype? ✔✔P1, P, Pk What phenotype can produce anti-PP1Pk? ✔✔p This antigen is found more on cord cells than on adult cells? ✔✔i Which antibody is associated with PCH? ✔✔Anti-P Which of the following is true regarding the Diego antigens? ✔✔They are not destroyed by Enzymes Which of the following is false regarding anti-Wrb? 1. They are mostly IgG 2. Implicated in HTR 3. Mostly IgM 4. Implicated in HDN ✔✔Mostly IgM Which of the following is true regarding the Yt blood group system? 1. Antigens are destroyed by common enzymes 2. Not denatured by DTT 3. Antibodies are usually IgM 4. Antibodies are usually IgG ✔✔Antibodies are usually IgG Which of the following is true regarding the Xg blood group system? 1. Antibodies are enhanced by enzymes 2. Antibodies are IgM 3. Antibodies react in the AHG phase 4. Xga is clinically significant ✔✔Antibodies react in the AHG phase John Milton Hagen (JMH) antigens are dstroyed by proteolytic enzymes? True or False ✔✔True Cromer antibodies are usually: ✔✔IgG Which of the following is false regarding the Knops blood group system? 1. Antigens are resistant to ficin 2. Antibodies are not clinically significant 3. Antigens are on a complement glycoprotein 4. Antigens are destroyed by ficin ✔✔Antigens are destroyed by ficin Which of the following is true regarding the Colton system? 1. Antibodies are usually IgG 2. Not implicated in HTR 3. Antibodies are usually IgM 4. Not implicated in HDN ✔✔Antibodies are usually IgG What is unique about the Chido/Rodgers system? ✔✔Antibodies are neutralized by antigen positive plasma When is anti-SC3 produced in patients? ✔✔If pt is Scianna null phenotype Ina and Inb are destroyed by: 1. Papain 2. Ficin 3. DTT 4. All of the above ✔✔All of the above (papain, ficin, DTT) Which of the Gerbich phenotype produces anti-Ge2? ✔✔Ge:-2,3,4 How would and R1R1 person translate to Rosenfield terminology in the Rh system? ✔✔Rh: 1,2,-3,-4,5 (FR= DCe/DCe) Anti-C is found in an O negative patient going to surgery. What type of blood should you prepare for transfusion? ✔✔rr type O What antibody might you suspect in a patient of Polynesian heritage? ✔✔Anti-Jk3 A patient on antithymocyte globulin therapy may produce antibodies that mimic what specificity? ✔✔Lutheran Which of the following Fisher-Race phenotypes compares with Rosenfield's Rh:1,-2,3,4,-5 ✔✔R2R2 (DcE/DcE) A patient has formed anti-KL. What patient population's RBCs might be compatible? ✔✔McLeod persons Anti-rhi can react with what phenotype? ✔✔R1R2 Which cells would be devoid of glycophroin B? ✔✔Mk/Mk What is unique about the In(JK) gene? ✔✔Resistant to lysis in 2M urea This Fy antigen is not altered by ficin or papain? ✔✔Fy3 A patient whose red cells types as D+C-E-c-e- Evans + could form what antibody? ✔✔Anti-Hro What does rh' represent in the Weiner nomenclature? ✔✔C Which of the following may produce anti-Rh29? ✔✔Rh null person Cellano would have strong expression in all of the following EXCEPT: a. K-k+ Kp(a+b+) b. K+k+ Kp(a-b+) c. K-k+ Kp(a-b+) d. K-k+ Kp(a+b-) ✔✔K-k+Kp(a+b-) (due to Kpa effect) What may be the best source of Lewis null cells? ✔✔Cord blood What antibody may be made from Ko indivduals? ✔✔Anti-Ku The cde/cde genotype has the least amount of what antigen? ✔✔LW Which of the following represents a null phenotype? 1. INAB 2. Fy5 3. SC1 4. Hy ✔✔INAB What is true concerning Rh null cells? 1. They are negative for Jk3 2. They are negative for Jk5 3. They are negative for S 4. All of the above ✔✔They are negative for Jk5 What Rh antibody is typically discribed as alloanti-e like? ✔✔Anti-hrB Which RH antigen is high frequency? 1. Bea 2. Evans 3. hrH 4. HrB ✔✔HrB Which of the following represent decreasing immunogenicity for major Rh antigens? ✔✔D>c>E>C>e Which MNS antigen is representative of a anti-Lepore hybrid? ✔✔Henshaw Ficin treatment would be helpful in which of the following? 1. Anti-M 2. Anti-Doa 3. Anti-Lu14 4. Anti-Ch ✔✔Anti-Doa Anti-N' will react with what cells? ✔✔N+,N= What can be said about the dominant type Lu(a-b-) person who inherits two normal Lub genes? ✔✔Anti-Lub can be adsorbed and eluted from their red blood cells What antigens are denatured upon performing and elution with glycine EDTA? ✔✔Kell and Era This antibody is in the MNS group and is reactive with HUT+ RBCs. ✔✔Mia Treating RBCs with this substance could render them Dombrock neg. ✔✔DTT This Rh antigen is a low incidence antigen associated with partial D phenotype DFR? ✔✔FPTT This high frequency antigen is antithetical to Lu9? ✔✔Lu6 The high frequency antigen Santini(Kell system) in antithetical to? ✔✔Callais The Me antigen is expressed on ______ and part of the ______ system? ✔✔GPB/MNS This phenotype expresses Gya, Hy, and Joa? ✔✔DOYA-neg A 56 yr old male who is type O received a stem cell transplant from an allogenic A donor. At day 128 post transplant the recipients isoagglutinins are no longer demonstrable. What type of plasma should the patient receive? ✔✔A What antibody is suspected when all 12 panel cells are reactive at AHG phase and reactions are unaffected by enzymes, DTT, and AET? ✔✔Antibody against Gill A patient with a phenotype K-k+Kp(a+b-) would have a weakened: ✔✔k antigen