National Phlebotomy Certification Exam
Questions and Answers with Certified
Solutions
An individual who has little resistance to an infectious microbe is referred to as a susceptible
what? ✔✔Host
Which of the follow
...
National Phlebotomy Certification Exam
Questions and Answers with Certified
Solutions
An individual who has little resistance to an infectious microbe is referred to as a susceptible
what? ✔✔Host
Which of the following is an example of a work practice control that reduces risk of exposure to
bloodborne pathogens? ✔✔Wearing gloves to draw blood
This ion is essential to the coagulation process. ✔✔Calcium
QC protocols prohibit use of outdated evacuated tubes because why? ✔✔Tubes my not fill
completely, changing additive-to-sample ratios.
Molecular genetic testing requires what? ✔✔Specimens to be collected in sterile EDTA.
What is the purpose of an antiglycolytic agent? ✔✔Preserve glucose
Which on of the following tubes is filled first when multiple tubes are filled using an evacuated
tube system? ✔✔Blood culture (SPS) tube
What is the recommended disinfectant for blood culture sites in infants 2 months and older?
✔✔Chlorhexidine gluconate
Latent fibrin formation in serum can result from what? ✔✔Incomplete clotting when centrifuged
In which of the following areas does capillary specimen collection differ from routine
venipuncture when collecting a BUN and CBC? ✔✔Order of draw
This is an example of a preanalytical error made at the time of collection. ✔✔Failing to mix
tubes
What constitutes a positive modified Allen test? ✔✔The hand color returns to normal in 15
seconds.
According to CLSI, which tubes should be placed upright as soon as they are mixed?
✔✔Nonanticoagulant gel tubes
Reference values are higher for capillary specimens with what test? ✔✔Glucose
According to CLSI, the maximum time limit for separating serum or plasma from cells is how
long? ✔✔2.0 hours from time of collection
All of the following are a reason for transporting tubes with the stoppers up EXCEPT which of
the following? ✔✔Maintains the sterility of the sample.
The liquid portion of a clotted specimen is called what? ✔✔Serum
The most common reason for glucose monitoring through POCT is to what? ✔✔Monitor glucose
levels for diabetic care.
The AMT, ACA, and ASCP are agencies that do what? ✔✔Certify laboratory professionals
A quadriplegic patient can do what of the following? ✔✔Can feel pain if there is no sensory
damage.
The standard of care used in phlebotomy malpractice cases is often based on guidelines from
what organization? ✔✔CLSI - Clinical and Laboratory Standards Institute
In collecting blood cultures, one of the most frequent errors made is what? ✔✔Improper
cleansing of the collection site
Which type of specimen is typically used for routine urinalysis? ✔✔Random
A positive FIT test indicates what? ✔✔Bleeding in the colon or rectum
Accessioning a specimen involves what? ✔✔AOTA-Connecting it with the correct individual,
giving it a unique identification number, and recording it in the order it was received.
A blood specimen collected in nongel tube has been centrifuged. The cells at the bottom are free
flowing and can be resuspended if the tube is inverted. The term used to describe the liquid
portion on top of the cells is what? ✔✔Plasma
The composition of capillary puncture blood more closely resembles what? ✔✔Arterial Blood
A phlebotomist arrives to collect a 2-hour post-prandial glucose specimen on an inpatient and
discovers that 2 hours have not elapsed since the patient's meal. What should the phlebotomist
do? ✔✔Ask the patient's nurse to verify the correct time to draw the specimen.
Which of the following is referred to as a delivering chamber of the heart? ✔✔Left ventricle
According to laboratory policy, specimens collected need to yield 2.5 times the amount of
sample needed to perform the test. If a chemistry test requires 0.5mL of serum, which of the
following tubes is the smallest that can be used? ✔✔3-mL red top
The main difference between serum and plasma is what? ✔✔Plasma has fibrinogen, serum does
not.
Which of the following represents proper collection and handling of newborn screening
specimens? ✔✔Use one free-flowing drop to fill both sides; air-dry flat and elevated.
Which of the following is a safe area for infant heel puncture? ✔✔Medial plantar surface
Generally, the minimum force and time required to result in good separation of serum or plasma
from the cells is what? ✔✔1,000 g for 10 minutes
All of the following statements concerning an employee bloodborne pathogen exposure incident
are true EXCEPT what? ✔✔The source patient must submit to HIV and HBV testing within 48
hours.
Which additive prevents coagulation by binding calcium? ✔✔Potassium EDTA
A prothrombin specimen must be collected from a patient with IVs in both arms. The best place
to collect the specimen is from where? ✔✔Below on of the IVs
Biological Substance Category B regulations must be followed when shipping laboratory
specimens for diagnostic purposes by public transportation. These regulations require the
specimens to be what? ✔✔Triple packaged in a watertight primary container.
Which of the following microcollection containers should be filled first if collected by capillary
puncture? ✔✔Lavender top
Which of the following specimens is negatively affected by chilling? ✔✔Potassium
The CAP requires QC for many waived tests to be performed when? ✔✔When test manufacturer
specifies.
A technologist asks the phlebotomist to collect 5cc of whole blood for a special test. What
volume tube should be used? ✔✔5 mL
Transporting tubes with the stopper up has nothing to do with what? ✔✔DOES NOT
MAINTAIN THE STERILITY OF THE SAMPLE!
The first tube of cerebrospinal fluid (CSF) collected is typically used for what? ✔✔Chemistry
studies
The manufacturer must supply an SDS for which one? ✔✔Isopropyl and methyl alcohol.
The abbreviation for the federal agency that instituted and enforces regulations requiring the
labeling of hazardous materials is what? ✔✔OSHA
What is NAACLS? ✔✔National Accrediting Agency for Clinical Laboratory Sciences -
developed the standardized educational curriculum with a recognized body of knowledge, skills,
and standards of practice for the phlebotomy profession.
What is CLIA? ✔✔Clinical Laboratory Improvement Amendments
TJC ✔✔The Joint Commission - independent, not for profit organization charged with, among
other things, establishing standards for the operation of hospitals and other health related
facilities and services.
NPSG ✔✔National Patient Safety Goals
CAP ✔✔College of American Pathologists - not for profit organization for professionals in the
field of laboratory medicine. Offers proficiency testing and a continuous form of laboratory
inspection by a team made up of pathologists and laboratory managers.
CLIAC ✔✔Clinical Laboratory Improvement Advisory Committee - to provide technical and
scientific advice and guidance to the appropriate people in CMS.
NAACLS ✔✔National Accrediting Agency for Clinical Laboratory Sciences - an authority on
quality clinical laboratory education.
CLSI ✔✔Clinical and Laboratory Standard Institute - organization that develops clinical and
laboratory practices and promotes their uses worldwide.
NHSN ✔✔National Healthcare Safety Network - provides data on healthcare associated
infections.
HICPAC ✔✔Healthcare Infection Control Practices Advisory Committee - provides the CDC
with advice and guidance regarding the practice of infection control and prevention in healthcare
settings.
CDC ✔✔Centers for Disease Control - investigates and controls various diseases.
NIOSH ✔✔National Institute for Occupational Safety and Health - part of the CDC, responsible
for conducting research and making recommendations for the prevention of work related illness
and injury.
OSHA ✔✔Occupational Safety and Health Administration - mandates and enforces safe
working conditions for employees.
HCS ✔✔Hazard Communication Standard - HazCom - right to know.
First tube
Culture - Cinnamon ✔✔Yellow - Microbiology - Blood Culture - SPS Additive - Bacteremia or
septicemia
2 bottles - Anaerobic and aerobic (butterfly needle-collect aerobic first) No capillary stick. 2 sets
collect 30 minutes apart from different arms.
Second tube
Citrate - Can ✔✔Light Blue - Coagulation - Sodium Citrate - PT/INR, PTT, APTT, D-dimer,
9:1, butterfly-throw first tube away
Orange-Chemistry-Thrombin-Coagulation
Black-Chemistry-Sodium Citrate- ESR-4:1
Third tube
Serum - Surely ✔✔Red-Chemistry/Blood Bank-No Additive-Type&Xmatch
Tiger-Chemistry-No Additive-STDs, HIV, HCG, Drugs, BMP, Lipid, T3-T4, Antibodies, FBS,
Liver/Kidney issues
Gold-Chemistry-No Additive- Same tests as Tiger
Fourth tube
Heparin - Help ✔✔Light Green-Chemistry-Lithium-Inhibits thrombin-PST, BMP, Stat
electrolytes, Plasma
Green-Chemistry-Sodium-Inhibits thrombin-Tropin, Lactic acid on ice, Stat electrolytes
Fifth tube
EDTA - Eliminate ✔✔Lavender-Hematology-EDTA-CBC, Malaria, A1C, ABROH, Blood type,
DAT, ESR, Retic, Mono, Hemoglobin
Ammonia-Chemistry- Delivered on ice
Sixth tube
Sodium Fluoride/Potassium Oxalate - Odors ✔✔Gray-Chemistry-Sodium Fluoride/Potassium
Oxalate-2hr post, ETOH, GTT, Fasting BS, Antiglycolytic (glucose tolerance)
Seventh tube
ACD ✔✔Yellow-Blood Bank/Immunohematology-ACD-DNA, HLA, Organ Transplant, Bone
Marrow match
Royal Blue-By Itself
EDTA, None, Sodium Heparin ✔✔Royal Blue-Chemistry-Trace Free/EDTA/Sodium HeparinHeavy Metals-aluminum, arsenic, copper, lead, iron, zinc
Which of the following specimens would most likely be rejected for testing? ✔✔A hemolyzed
potassium specimen.
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