Microbiology > CASE SOLUTIONS > Methods in Molecular Biology 1469 Chromosome and Genomic Engineering in Plants (All)
Methods in Molecular Biology 1469 Chromosome and Genomic Engineering in Plants
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Transformation or transfection is an indispensable tool in basic and applied studies in biological sciences. In plants, a number of species can be transformed by an Agrobacteriummediated system, parti... cle bombardment, and/or protoplast fusion. Compared to other organisms, however, these three techniques are uncontrollable with regard to the insertion of exogenous genes or DNA because the insertion into the genome or chromosome is quite random, and multiple-copy insertion frequently occurs. This random and multiple-copy insertion increases the risk of disrupting essential genes. To avoid such risk, gene targeting via homologous recombination is most desirable, as has been shown in yeast and mice. However, the occurrence of homologous recombination is quite limited in plants, except for in some lower plants (i.e., Physcomitrella patens and Chlamydomonas reinhardtii). [Show More]
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