Biology > STUDY GUIDE > University of Pennsylvania - BIOL 204BIOL 204 (2018) Problem Set 3 (All)

University of Pennsylvania - BIOL 204BIOL 204 (2018) Problem Set 3

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Biology 204 Spring 2018/REA 1 Problem Set 3: Enzyme kinetics and mechanism Questions: Question 4 provides you with an introduction to the pros and cons of the linear transformations that are mos... t commonly employed for estimating Km and Vmax (if not in the real world, in textbooks and MCATs, GREs and the like). In working your way through this question, you will discover some of the merits and inadequacies of these transformations for yourself. Questions 6, 7 and 8 have their origins in research that was actually conducted except that the scenario as it plays out in question 6 have a slightly different outcome, and the entities designated ‘cystione’, ‘cadstatins’, and ‘CS synthase’ are a fiction, albeit a fiction that has a rational basis. Questions 7 and 8 provide you with concrete, real-world examples of how Michaelis-Menten kinetics might be applied to bisubstrate reactions of which the former deals with the discovery of a drugs that revolutionized the treatment and prevention of atherosclerosis, the latter with the development of the most successful herbicide in history. Question 9 illustrates the kinetic basis of the efficacy of ACE inhibitors for the treatment of hypertension, which rank among the most widely prescribed drugs in the United States and Europe. 1. An enzyme catalyzes a reaction at a velocity of 20 μmol/min when the concentration of substrate (S) is 0.01 M. The Km for this substrate is 1 × 10-5 M. Assuming that Michaelis-Menten kinetics are followed, what will the reaction velocity be when the concentration of S is [Show More]

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