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Virginia Highlands Community College - BIOLOGY 101DNA_Sequence_Assembly_click_learn_worksheet( all answers correct)

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Howard Hughes Medical Institute 2010 Holiday Lectures on Science DNA Sequence Assembly—Student Worksheet Answer the following questions as you proceed through the slides. 1. Read the information... on slide 1 and watch the video clip. Explain what the amount of DNA sequence that can be generated for a dollar reveals about sequencing technology? _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ 2. Summarize how Sanger sequencing works. _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ 3. What is the upper limit for the number of nucleotides that can be read using Sanger sequencing? Explain why this is the limit. _____________________________________________________________________________________ _____________________________________________________________________________________ 4. Read slide 4 and provide an example of an overlap sequence in the space below. About This Worksheet This worksheet complements the Click and Learn “DNA Sequence Assembly” developed in conjunction with the 2010 Holiday Lectures on Science, “Viral Outbreak: The Science of Emerging Disease.” Author: Ann Brokaw, Rocky River High School, Rocky River, OHPage 2 of 3 DNA Sequence Assembly—Student Worksheet 5. Using your own words, describe shotgun sequencing. _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ 6. Describe deep sequencing. _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ 7. Read slides 7 through 16 and answer the following questions as you proceed through the slides. a. How is overlapping text used in this example? _____________________________________________________________________________________ _____________________________________________________________________________________ b. Explain in your own words what it means to “align” text or sequence. (You may use an example if it helps.) [Show More]

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