Howard Hughes Medical Institute
2010 Holiday Lectures on Science
DNA Sequence Assembly—Student Worksheet
Answer the following questions as you proceed through the slides.
1. Read the information on slide 1 and watch
...
Howard Hughes Medical Institute
2010 Holiday Lectures on Science
DNA Sequence Assembly—Student Worksheet
Answer the following questions as you proceed through the slides.
1. Read the information on slide 1 and watch the video clip. Explain what the amount of DNA
sequence that can be generated for a dollar reveals about sequencing technology?
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2. Summarize how Sanger sequencing works.
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3. What is the upper limit for the number of nucleotides that can be read using Sanger sequencing?
Explain why this is the limit.
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4. Read slide 4 and provide an example of an overlap sequence in the space below.
About This Worksheet
This worksheet complements the Click and Learn “DNA Sequence Assembly”
developed in conjunction with the 2010 Holiday Lectures on Science, “Viral
Outbreak: The Science of Emerging Disease.”
Author: Ann Brokaw, Rocky River High School, Rocky River, OHPage 2 of 3 DNA Sequence Assembly—Student Worksheet
5. Using your own words, describe shotgun sequencing.
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6. Describe deep sequencing.
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7. Read slides 7 through 16 and answer the following questions as you proceed through the slides.
a. How is overlapping text used in this example?
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b. Explain in your own words what it means to “align” text or sequence. (You may use an
example if it helps.)
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