Engineering > SOLUTIONS MANUAL > George Washington University_ECE 3820_unit_9_ps1_ligand_binding_answer_key-REVIEWED BY EXPERTS 2021 (All)
George Washington University_ECE 3820_unit_9_ps1_ligand_binding_answer_key-REVIEWED BY EXPERTS 2021
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PROBLEM SET 9.1: LIGAND BINDING ANSW ER KEY 1. The specific ryanodine binding to isolated cardiac sarcoplasmic reticulum was determ ined and gave the following results: Bound Ryanodine Free Ryanod... ine (pmol/mg) (nM) 0.31 0.19 0.73 0.52 1.23 1.27 1.67 2.08 2.01 2.99 2.53 7.47 2.82 17.18 3.12 26.88 W hat is the B max and Kd for ryanodine binding? Is the binding cooperative? Be sure to include any graphs that you might want to make. Mathematics without the graphics is a bad mistake, because the human mind is a much better filter than mathematical constructs like the correlation coefficient. Here we calculate B/F and construct a Scatchard plot. The Data is in the Table 9.PS1.1 below: Table 9.PS1.1. Values for the Scatchard Plot Bound Ryanodine (pmol mg-1) Free Ryanodine ( nM) Bound/Free (pmol mg-1nM-1) 0.31 0.19 1.63 0.73 0.52 1.40 1.23 1.27 0.97 1.67 2.08 0.80 2.01 2.99 0.67 2.53 7.47 0.34 2.82 17.18 0.16 3.12 26.88 0.11 [Show More]
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