TK01 Keeping a Lab Notebook Microbiology Lab1
Course Objectives:
Cultivation of samples (growth conditions) - equipment used Identification of samples (biochemical assays) - tests available
Evaluation of samples (micr
...
TK01 Keeping a Lab Notebook Microbiology Lab1
Course Objectives:
Cultivation of samples (growth conditions) - equipment used Identification of samples (biochemical assays) - tests available
Evaluation of samples (microscopy) - visualizations and recognitions key concepts
Basic Equipment:
1. Cleaning: autoclave 125 ° C, steamed, heat and pressure sterilization, chamber with thick door, not dry air but steamed - more efficient, make sure depressurized before opening
Time restrictions: no autoclave then use steam (minutes) vs. hot air (hours)
2. Growing: fixed incubator = 37 ° C; shaker incubator = 37 ° C, for growth mediator, rotates and shakes in circular motions to aerate and help optimize bacterial growth
3. Visualizing: Microscopy, can't see with just the naked eye, must know parts of microscope
4. Storing: Refrigerate at 4 ° C, slows bacterial growth, prolongs life of sample at this temperature, when not finished with experimentation
Lab Safety:
1. Never eat or drink in lab - contamination risks
2. Wear Personal Protective Equipment (PPE):
Use gloves: latex, nitrile (hypoallergenic) purple,blue or green, cold thermal - liquid nitrogen, etc., heat thermal resistant gloves - extreme hot conditions
Eyewear protection - goggles
Lab coat - dependent on working with materials that may be spilt on clothes
3. NEVER leave lab while wearing PPE includes: Public Hallway, Restroom, Cafeteria
Lab Notebook:
First Name Initial, Last name initial, Experiment #, Title of Experiment Example: TK01 Keeping a Lab Notebook
Objective: Developing an organized lab notebook for documenting experiments, data, procedures and records.
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